Implementation of a Multiplex rRT-PCR for Zika, Chikungunya, and Dengue Viruses: Improving Arboviral Detection in an Endemic Region

Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2020-03, Vol.102 (3), p.625-628
Main Authors: Cantero, César, Cardozo, Fátima, Waggoner, Jesse J, Pinsky, Benjamin A, Espínola, Anibal, Infanzón, Belén, Acosta, María Eugenia, Aria, Laura, Arévalo de Guillén, Yvalena, Cuevas, Teresa, Rojas, Vicenta, Segovia, Clotilde, Centurión, Ana, Rojas, Alejandra
Format: Article
Language:English
Subjects:
Adolescent
Adult
Chikungunya Fever - diagnosis
Chikungunya Fever - epidemiology
Chikungunya virus
Child
Dengue - diagnosis
Dengue - epidemiology
Dengue fever
Endemic Diseases
Female
Humans
Male
Middle Aged
Multiplex Polymerase Chain Reaction - methods
Paraguay - epidemiology
Reverse Transcriptase Polymerase Chain Reaction - methods
Young Adult
Zika virus
Zika Virus Infection - diagnosis
Zika Virus Infection - epidemiology
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Summary:Arboviral diagnosis has been complicated throughout the tropical and subtropical Americas by the recent co-circulation of Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV). The aim of this study was to implement a multiplex real-time RT-PCR (rRT-PCR) for ZIKV, CHIKV, and DENV in Paraguay to test patients who were clinically suspected of having dengue. We tested 110 sera from patients who presented to the Hospital de Clínicas in 2016 and had testing for DENV nonstructural protein 1 (NS1; 40 positive and 70 negative). Using a composite reference standard, we confirmed 51 dengue cases (46.4%): 38/40 NS1 positive and 13/70 NS1 negative. Chikungunya virus and ZIKV were detected in one sample each, both were DENV NS1 negative. The NS1 test demonstrated good agreement with rRT-PCR for DENV. However, multiplex rRT-PCR identified a subset of dengue cases and additional arboviral infections that would not be detected if NS1 assays are relied upon for diagnosis.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.19-0707