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High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age
Aim To reveal whether there are differences in follicular fluid metabolomics profile of women with advanced maternal age (AMA). Method The group with advanced maternal age includes 23 patients above the age of 40, and the control group includes 31 patients aged between 25 and 35 years and AMH values...
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| Published in: | Journal of assisted reproduction and genetics 2020-02, Vol.37 (2), p.321-330 |
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| Main Authors: | , , , , |
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| cites | cdi_FETCH-LOGICAL-c296x-7a7cd096671d39026dbab51f02330a82321f1ab22d6b0aac3b15b9f5d1cac9273 |
| container_end_page | 330 |
| container_issue | 2 |
| container_start_page | 321 |
| container_title | Journal of assisted reproduction and genetics |
| container_volume | 37 |
| creator | Dogan, B. Karaer, A. Tuncay, G. Tecellioglu, N. Mumcu, A. |
| description | Aim
To reveal whether there are differences in follicular fluid metabolomics profile of women with advanced maternal age (AMA).
Method
The group with advanced maternal age includes 23 patients above the age of 40, and the control group includes 31 patients aged between 25 and 35 years and AMH values above 1.1 ng/mL with no low ovarian response history. A single follicular fluid sample from a MII oocyte obtained during the oocyte pick-up procedure was analyzed with high-resolution
1
H-NMR (nuclear magnetic resonance) spectroscopy. The results were evaluated using advanced bioinformatics analysis methods.
Results
Statistical analysis of the NMR spectroscopy data from two groups showed that α-glucose and β-glucose levels of follicular fluid were decreased in the patients with AMA, while in contrast, lactate and trimethylamine N-oxide (TMAO) levels were increased in these patients compared with the controls. In addition to these, there was an increase in alanine levels and a decrease in acetoacetate levels in patients with AMA. However, these changes were not statistically significant.
Conclusion
Obtained results suggest that the follicular cell metabolism of patients with AMA is different from controls. These environmental changes could be associated with the low success rates of IVF treatment seen in these patients. |
| doi_str_mv | 10.1007/s10815-020-01693-x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7056815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2339792036</sourcerecordid><originalsourceid>FETCH-LOGICAL-c296x-7a7cd096671d39026dbab51f02330a82321f1ab22d6b0aac3b15b9f5d1cac9273</originalsourceid><addsrcrecordid>eNp9kc2KFDEUhQtRnB99AVcBN26iN8lUpbMRZBjtgVFBdB1u5ac7Q6pSJlU9Pa_gU5u2B0UXrhKS7x7OuadpXjB4zQDkm8JgxVoKHCiwTgm6f9ScslYKKoWAx_UO7YrCRbc6ac5KuQUAteLiaXMimLrgXSdPmx_rsNnS7EqKyxzSSNiafvr4hZTJmTmnYtJ0T8Jog8HZFbLDHPDAlfpIBjdjn2IagilkysmH6EjyxKcYg1kiZuLjEizxOQ3kLg1uJHdh3hK0OxyNs2SoqnnESHDjnjVPPMbinj-c582391dfL9f05vOH68t3N9Rw1e2pRGksqOqeWaGAd7bHvmUeeA2NNR9nnmHPue16QDSiZ22vfGuZQaO4FOfN26PutPSDs8aNc8aopxwGzPc6YdB__4xhqzdppyW0XV14FXj1IJDT98WVWQ-hGBcjji4tRVcjSioOoqvoy3_Q27QcAh8oyWoNirNK8SNl6sZLdv63GQb6ULU-Vq1r1fpX1Xpfh8RxqFR43Lj8R_o_Uz8BJuGukg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2371194921</pqid></control><display><type>article</type><title>High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age</title><source>PubMed (Medline)</source><source>Springer Link</source><creator>Dogan, B. ; Karaer, A. ; Tuncay, G. ; Tecellioglu, N. ; Mumcu, A.</creator><creatorcontrib>Dogan, B. ; Karaer, A. ; Tuncay, G. ; Tecellioglu, N. ; Mumcu, A.</creatorcontrib><description>Aim
To reveal whether there are differences in follicular fluid metabolomics profile of women with advanced maternal age (AMA).
Method
The group with advanced maternal age includes 23 patients above the age of 40, and the control group includes 31 patients aged between 25 and 35 years and AMH values above 1.1 ng/mL with no low ovarian response history. A single follicular fluid sample from a MII oocyte obtained during the oocyte pick-up procedure was analyzed with high-resolution
1
H-NMR (nuclear magnetic resonance) spectroscopy. The results were evaluated using advanced bioinformatics analysis methods.
Results
Statistical analysis of the NMR spectroscopy data from two groups showed that α-glucose and β-glucose levels of follicular fluid were decreased in the patients with AMA, while in contrast, lactate and trimethylamine N-oxide (TMAO) levels were increased in these patients compared with the controls. In addition to these, there was an increase in alanine levels and a decrease in acetoacetate levels in patients with AMA. However, these changes were not statistically significant.
Conclusion
Obtained results suggest that the follicular cell metabolism of patients with AMA is different from controls. These environmental changes could be associated with the low success rates of IVF treatment seen in these patients.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/s10815-020-01693-x</identifier><identifier>PMID: 31942667</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Age ; Alanine ; Assisted Reproduction Technologies ; Bioinformatics ; Environmental changes ; Follicular fluid ; Gynecology ; Health risk assessment ; Human Genetics ; Lactic acid ; Magnetic resonance spectroscopy ; Medicine ; Medicine & Public Health ; Metabolomics ; NMR ; Nuclear magnetic resonance ; Reproductive Medicine ; Spectrum analysis ; Statistical analysis ; Trimethylamine</subject><ispartof>Journal of assisted reproduction and genetics, 2020-02, Vol.37 (2), p.321-330</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Journal of Assisted Reproduction and Genetics is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c296x-7a7cd096671d39026dbab51f02330a82321f1ab22d6b0aac3b15b9f5d1cac9273</citedby><cites>FETCH-LOGICAL-c296x-7a7cd096671d39026dbab51f02330a82321f1ab22d6b0aac3b15b9f5d1cac9273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056815/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056815/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Dogan, B.</creatorcontrib><creatorcontrib>Karaer, A.</creatorcontrib><creatorcontrib>Tuncay, G.</creatorcontrib><creatorcontrib>Tecellioglu, N.</creatorcontrib><creatorcontrib>Mumcu, A.</creatorcontrib><title>High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><description>Aim
To reveal whether there are differences in follicular fluid metabolomics profile of women with advanced maternal age (AMA).
Method
The group with advanced maternal age includes 23 patients above the age of 40, and the control group includes 31 patients aged between 25 and 35 years and AMH values above 1.1 ng/mL with no low ovarian response history. A single follicular fluid sample from a MII oocyte obtained during the oocyte pick-up procedure was analyzed with high-resolution
1
H-NMR (nuclear magnetic resonance) spectroscopy. The results were evaluated using advanced bioinformatics analysis methods.
Results
Statistical analysis of the NMR spectroscopy data from two groups showed that α-glucose and β-glucose levels of follicular fluid were decreased in the patients with AMA, while in contrast, lactate and trimethylamine N-oxide (TMAO) levels were increased in these patients compared with the controls. In addition to these, there was an increase in alanine levels and a decrease in acetoacetate levels in patients with AMA. However, these changes were not statistically significant.
Conclusion
Obtained results suggest that the follicular cell metabolism of patients with AMA is different from controls. These environmental changes could be associated with the low success rates of IVF treatment seen in these patients.</description><subject>Age</subject><subject>Alanine</subject><subject>Assisted Reproduction Technologies</subject><subject>Bioinformatics</subject><subject>Environmental changes</subject><subject>Follicular fluid</subject><subject>Gynecology</subject><subject>Health risk assessment</subject><subject>Human Genetics</subject><subject>Lactic acid</subject><subject>Magnetic resonance spectroscopy</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolomics</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Reproductive Medicine</subject><subject>Spectrum analysis</subject><subject>Statistical analysis</subject><subject>Trimethylamine</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc2KFDEUhQtRnB99AVcBN26iN8lUpbMRZBjtgVFBdB1u5ac7Q6pSJlU9Pa_gU5u2B0UXrhKS7x7OuadpXjB4zQDkm8JgxVoKHCiwTgm6f9ScslYKKoWAx_UO7YrCRbc6ac5KuQUAteLiaXMimLrgXSdPmx_rsNnS7EqKyxzSSNiafvr4hZTJmTmnYtJ0T8Jog8HZFbLDHPDAlfpIBjdjn2IagilkysmH6EjyxKcYg1kiZuLjEizxOQ3kLg1uJHdh3hK0OxyNs2SoqnnESHDjnjVPPMbinj-c582391dfL9f05vOH68t3N9Rw1e2pRGksqOqeWaGAd7bHvmUeeA2NNR9nnmHPue16QDSiZ22vfGuZQaO4FOfN26PutPSDs8aNc8aopxwGzPc6YdB__4xhqzdppyW0XV14FXj1IJDT98WVWQ-hGBcjji4tRVcjSioOoqvoy3_Q27QcAh8oyWoNirNK8SNl6sZLdv63GQb6ULU-Vq1r1fpX1Xpfh8RxqFR43Lj8R_o_Uz8BJuGukg</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Dogan, B.</creator><creator>Karaer, A.</creator><creator>Tuncay, G.</creator><creator>Tecellioglu, N.</creator><creator>Mumcu, A.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200201</creationdate><title>High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age</title><author>Dogan, B. ; Karaer, A. ; Tuncay, G. ; Tecellioglu, N. ; Mumcu, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c296x-7a7cd096671d39026dbab51f02330a82321f1ab22d6b0aac3b15b9f5d1cac9273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Age</topic><topic>Alanine</topic><topic>Assisted Reproduction Technologies</topic><topic>Bioinformatics</topic><topic>Environmental changes</topic><topic>Follicular fluid</topic><topic>Gynecology</topic><topic>Health risk assessment</topic><topic>Human Genetics</topic><topic>Lactic acid</topic><topic>Magnetic resonance spectroscopy</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolomics</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Reproductive Medicine</topic><topic>Spectrum analysis</topic><topic>Statistical analysis</topic><topic>Trimethylamine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dogan, B.</creatorcontrib><creatorcontrib>Karaer, A.</creatorcontrib><creatorcontrib>Tuncay, G.</creatorcontrib><creatorcontrib>Tecellioglu, N.</creatorcontrib><creatorcontrib>Mumcu, A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dogan, B.</au><au>Karaer, A.</au><au>Tuncay, G.</au><au>Tecellioglu, N.</au><au>Mumcu, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><stitle>J Assist Reprod Genet</stitle><date>2020-02-01</date><risdate>2020</risdate><volume>37</volume><issue>2</issue><spage>321</spage><epage>330</epage><pages>321-330</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><abstract>Aim
To reveal whether there are differences in follicular fluid metabolomics profile of women with advanced maternal age (AMA).
Method
The group with advanced maternal age includes 23 patients above the age of 40, and the control group includes 31 patients aged between 25 and 35 years and AMH values above 1.1 ng/mL with no low ovarian response history. A single follicular fluid sample from a MII oocyte obtained during the oocyte pick-up procedure was analyzed with high-resolution
1
H-NMR (nuclear magnetic resonance) spectroscopy. The results were evaluated using advanced bioinformatics analysis methods.
Results
Statistical analysis of the NMR spectroscopy data from two groups showed that α-glucose and β-glucose levels of follicular fluid were decreased in the patients with AMA, while in contrast, lactate and trimethylamine N-oxide (TMAO) levels were increased in these patients compared with the controls. In addition to these, there was an increase in alanine levels and a decrease in acetoacetate levels in patients with AMA. However, these changes were not statistically significant.
Conclusion
Obtained results suggest that the follicular cell metabolism of patients with AMA is different from controls. These environmental changes could be associated with the low success rates of IVF treatment seen in these patients.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>31942667</pmid><doi>10.1007/s10815-020-01693-x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1058-0468 |
| ispartof | Journal of assisted reproduction and genetics, 2020-02, Vol.37 (2), p.321-330 |
| issn | 1058-0468 1573-7330 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7056815 |
| source | PubMed (Medline); Springer Link |
| subjects | Age Alanine Assisted Reproduction Technologies Bioinformatics Environmental changes Follicular fluid Gynecology Health risk assessment Human Genetics Lactic acid Magnetic resonance spectroscopy Medicine Medicine & Public Health Metabolomics NMR Nuclear magnetic resonance Reproductive Medicine Spectrum analysis Statistical analysis Trimethylamine |
| title | High-resolution 1H-NMR spectroscopy indicates variations in metabolomics profile of follicular fluid from women with advanced maternal age |
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