In vitro response and gene expression of human retinal Müller cells treated with different anti-VEGF drugs

•Human retinal Müller cells (MIO-M1) were treated with 4 different anti-VEGF drugs.•Cultures had decreased metabolism, viability and mitochondrial membrane potential.•Treatment led to higher levels of ROS and apoptosis.•Altered expression of angiogenesis, pro-apoptosis and inflammatory genes were fo...

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Bibliographic Details
Published in:Experimental eye research 2020-02, Vol.191, p.107903-107903, Article 107903
Main Authors: Cáceres-del-Carpio, Javier, Moustafa, M. Tarek, Toledo-Corral, Jaime, Hamid, Mohamed A., Atilano, Shari R., Schneider, Kevin, Fukuhara, Paula S., Costa, Rodrigo Donato, Norman, J. Lucas, Malik, Deepika, Chwa, Marilyn, Boyer, David S., Limb, G. Astrid, Kenney, M. Cristina, Kuppermann, Baruch D.
Format: Article
Language:English
Subjects:
Actins - genetics
Angiogenesis Inhibitors - pharmacology
Angiogenic Proteins - genetics
Apoptosis
Apoptosis Regulatory Proteins - genetics
Bevacizumab - pharmacology
Carrier Proteins - genetics
Cell Line
Cell Survival
Ependymoglial Cells - drug effects
Ependymoglial Cells - metabolism
Gene Expression - physiology
Glial Fibrillary Acidic Protein - genetics
Humans
Inflammation - genetics
Membrane Potential, Mitochondrial - physiology
Oxidative Stress - genetics
Ranibizumab - pharmacology
Reactive Oxygen Species - metabolism
Real-Time Polymerase Chain Reaction
Receptors, Vascular Endothelial Growth Factor
Recombinant Fusion Proteins - pharmacology
Vascular Endothelial Growth Factor A - antagonists & inhibitors
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Description
Summary:•Human retinal Müller cells (MIO-M1) were treated with 4 different anti-VEGF drugs.•Cultures had decreased metabolism, viability and mitochondrial membrane potential.•Treatment led to higher levels of ROS and apoptosis.•Altered expression of angiogenesis, pro-apoptosis and inflammatory genes were found.•Anti-VEGF drugs may contribute to cytotoxicity associated with chronic injections.
ISSN:0014-4835
1096-0007
1096-0007
DOI:10.1016/j.exer.2019.107903