Morphological characterization of domatium development in Callicarpa saccata

• Published in: Annals of botany 2020-03, Vol.125 (3), p.521-532
• Main Authors: Sarath, Emma, Ezaki, Kazune, Sasaki, Takenori, Maekawa, Yu, Sawada, Yuji, Hirai, Masami Yokota, Soejima, Akiko, Tsukaya, Hirokazu
• Format: Article
• Language: English
• Subjects: Animals; Ants; Callicarpa; Original; Plant Leaves; Symbiosis; X-Ray Microtomography
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mcz193

Domatia are plant structures within which organisms reside. Callicarpa saccata (Lamiaceae) is the sole myrmecophyte, or 'ant plant', that develops foliar (leaf-borne) myrmeco-domatia in this genus. In this work we examined domatium development in C. saccata to understand the developmental processes behind pouch-like domatia. Scanning electron microscopy, sectioning and microcomputed tomography were carried out to compare the leaves of C. saccata with those of the closely related but domatia-less myrmecophyte Callicarpa subaequalis, both under cultivation without ants. Callicarpa saccata domatia are formed as a result of excess cell proliferation at the blade/petiole junctions of leaf primordia. Blade/petiole junctions are important meristematic sites in simple leaf organogenesis. We also found that the mesophyll tissue of domatia does not clearly differentiate into palisade and spongy layers. Rather than curling of the leaf margins, a perturbation of the normal functioning of the blade/petiole junction results in the formation of domatium tissue. Excess cell proliferation warps the shape of the blade and disturbs the development of the proximal-distal axis. This process leads to the generation of distinct structures that facilitate interaction between C. saccata and ants.