Localization of PD‐L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles

Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr...

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Bibliographic Details
Published in:Journal of biophotonics 2020-03, Vol.13 (3), p.e201960034-n/a
Main Authors: Stepula, Elzbieta, König, Matthias, Wang, Xin‐Ping, Levermann, Janina, Schimming, Tobias, Kasimir‐Bauer, Sabine, Schilling, Bastian, Schlücker, Sebastian
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Biomarkers
Cancer
Cell death
Excitation
Full
Gold
gold nanoparticles
Image processing
Immunotherapy
Infrared lasers
Localization
Microscopy
Nanoparticles
PD-L1 protein
PD‐L1
Raman
SERS
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Summary:Programmed cell death‐ligand 1 (PD‐L1) is an important predictive biomarker. The detection of PD‐L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno‐SERS microscopy (iSERS) for localizing PD‐L1 on single cancer SkBr‐3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near‐infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False‐color iSERS images of the positive and negative controls clearly reveal the specific localization of PD‐L1 with SERS nanotag‐labeled antibodies. iSERS (SERS=surface‐enhanced Raman scattering) microscopy is an emerging Raman‐based staining technique for the selective localization of target proteins on cells and tissues using antibody‐SERS nanotag conjugates. In this contribution we demonstrate the feasibility of iSERS for imaging of programmed cell deathligand 1 (PD‐L1), an important predictive biomarker, on single SkBr‐3 breast cancer cells.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201960034