MicroRNA-19a-3p regulates cell growth through modulation of the PIK3IP1-AKT pathway in hepatocellular carcinoma

There are some controversies about the involvement of microRNA (miR)-19a-3p in hepatocellular carcinoma (HCC) biology, even though many studies have shown that it plays an important role in cancer. In this study, we found that miR-19a-3p is usually overexpressed in HCC tissues compared with correspo...

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Bibliographic Details
Published in:Journal of Cancer 2020-01, Vol.11 (9), p.2476-2484
Main Authors: Sun, Hai-Xiang, Yang, Zhang-Fu, Tang, Wei-Guo, Ke, Ai-Wu, Liu, Wei-Ren, Li, Yan, Gao, Chao, Hu, Bo, Fu, Pei-Yao, Yu, Min-Cheng, Gao, Bo-Wen, Shi, Ying-Hong, Fan, Jia, Xu, Yang
Format: Article
Language:English
Subjects:
Binding sites
Cell growth
Gene expression
Hypoxia
Liver cancer
Medical prognosis
Metastasis
MicroRNAs
Research Paper
Statistical analysis
Tumors
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Summary:There are some controversies about the involvement of microRNA (miR)-19a-3p in hepatocellular carcinoma (HCC) biology, even though many studies have shown that it plays an important role in cancer. In this study, we found that miR-19a-3p is usually overexpressed in HCC tissues compared with corresponding peritumorous tissues, and its expression was associated with tumor size and poor overall survival. MiR-19a-3p promoted cell proliferation significantly, and more cells were found in the S phase. In vivo, miR-19a-3p promoted liver tumor growth, and more HCC cells were found in the active cell cycle. Sequencing and bioinformatics analysis predicted that is a likely target gene of miR-19a-3p, and we next confirmed it by luciferase and rescue assays. Altogether, our data showed an important role of PIK3IP1 downregulation by miR-19a-3p in HCC progression, and the miR-19a-3p-PIK3IP1-AKT pathway may be a potential therapeutic target.
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.37748