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Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients

Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we de...

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Bibliographic Details
Published in:Scientific reports 2020-03, Vol.10 (1), p.4700-4700, Article 4700
Main Authors: Qiu, Chuanghua, Zhong, Lihong, Huang, Chunxiu, Long, Jia, Ye, Xuejun, Wu, Jingbo, Dai, Wenjie, Lv, Wei, Xie, Chongwei, Zhang, Junfang
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Language:English
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Summary:Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we developed a simple method to detect the level of cell-bound IgE by dissociating it from blood cells with lactic acid. Dissociated cell-bound IgE and plasma IgE levels were detected using the same ELISA kit at the same time. We established two clinical cohorts: an allergic patient group and a healthy participant group. In general, cell-bound IgE correlated well with plasma IgE; however, some patients exhibited high cell-bound IgE levels but low plasma IgE levels. We recommended 350 ng/mL peripheral blood total IgE (cell-bound IgE + plasma IgE) as the cut-off value for allergy diagnosis. Using this indicator, 90.32% of our allergic patients were correctly diagnosed. The peripheral blood total IgE level is a promising clinical diagnostic indicator in allergic patients and will provide more guidance for allergy diagnosis and therapeutic evaluation.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-61455-8