Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri

Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, OLL2809, a probiotic microorganism isolated from human feces, has the poten...

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Bibliographic Details
Published in:Nutrients 2020-02, Vol.12 (2), p.555
Main Authors: Mazzeo, Maria Fiorella, Luongo, Diomira, Sashihara, Toshihiro, Rossi, Mauro, Siciliano, Rosa Anna
Format: Article
Language:English
Subjects:
Animals
Bone marrow
Cellular Senescence - immunology
Contractility
Cytokines
Dendritic cells
Dendritic Cells - microbiology
Digestive system
Feces - microbiology
Homeostasis
Immune response
Immune system
Immunologic Factors - metabolism
Inflammation
Intestinal microflora
Intestine
Lactobacillus gasseri
Lipopolysaccharides
Lymphocytes
Lymphocytes T
Maturation
Mice
Microbiota
Microorganisms
Molecular modelling
Peptides
Pretreatment
Probiotics
Probiotics - pharmacology
Proteins
Proteomics
Secretome
T cell receptors
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Summary:Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, OLL2809, a probiotic microorganism isolated from human feces, has the potential to modulate various immune responses. The dendritic cells (DCs) are considered the main players in orchestrating the immune response, and their contact with intestinal microbiota is crucial for the development and homeostasis of gut immunity. To gain a perspective on the molecular mechanisms involved in the maturation process of DCs and investigate factors that could modulate these processes, a differential proteomic analysis was performed on the secretome of immature DCs, mature DCs (mDCs, induced by lipopolysaccharide (LPS)), and immature DCs challenged with OLL2809 before treatment with LPS (LGmDCs). The maturation process of DCs was associated to profound changes in the protein secretome and probiotic pre-treatment led to a dramatic modulation of several secreted proteins of mDC, not only classical immune mediators (i.e., cytokines, complement factors, T cell Receptor ligands) but also proteins involved in the contractile and desmosome machineries. The latter data highlight a novel mechanism by which can modulate the maturation process of DCs, reinforcing the concept of a protective anti-inflammatory role ascribed to this probiotic strain.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu12020555