Comparison of cytotoxic effects of calcium silicate-based materials on human pulp fibroblasts Mehmet

This study aimed to compare the in vitro cytotoxicity of Theracal LC, BiodentineTM, iRoot BP Plus, and MTA Angelus on human pulp fibroblasts (HPF). Fifteen discs from each calcium silicate-based material were prepared in sterile Teflon molds. After setting, the fabricated discs were eluated with a c...

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Published in:Journal of dental research, dental clinics, dental prospects dental clinics, dental prospects, 2019, Vol.13 (4), p.241-246
Main Authors: Adıgüzel, Mehmet, Ahmetoğlu, Fuat, Eldeniz, Ayçe Ünverdi, Tekin, Mehmet Gökhan, Göğebakan, Bülent
Format: Article
Language:English
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Summary:This study aimed to compare the in vitro cytotoxicity of Theracal LC, BiodentineTM, iRoot BP Plus, and MTA Angelus on human pulp fibroblasts (HPF). Fifteen discs from each calcium silicate-based material were prepared in sterile Teflon molds. After setting, the fabricated discs were eluated with a culture medium for 24 h. HPF cells were plated onto 24-well plates at 5×10 cells/well, and the cells were exposed to the material eluates. The cell viability was evaluated with MTT assay at three different times (24, 48, and 72 h). Data were statistically analyzed. The apoptotic/necrotic status of HPF cells exposed to material eluates was determined by flow cytometry. The differences between the effects of Theracal LC, Biodentine , MTA Angelus, and iRoot BP Plus on HPF cells were found to be statistically significant (P
ISSN:2008-210X
2008-2118
DOI:10.15171/joddd.2019.037