Translation of mouse model to human gives insights into periodontitis etiology

• Published in: Scientific reports 2020-03, Vol.10 (1), p.4892, Article 4892
• Main Authors: Nashef, Aysar, Matthias, Munz, Weiss, Ervin, Loos, Bruno G., Jepsen, Søren, van der Velde, Nathalie, Uitterlinden, André G., Wellmann, Jürgen, Berger, Klaus, Hoffmann, Per, Laudes, Matthias, Lieb, Wolfgang, Franke, Andre, Dommisch, Henrik, Schäfer, Arne, Houri-Haddad, Yael, Iraqi, Fuad A.
• Format: Article
• Language: English
• Subjects: 38; 38/39; 692/699/3017; 692/700; Aggressive Periodontitis - etiology; Aggressive Periodontitis - genetics; Animals; Association analysis; Bone growth; Chronic Periodontitis - etiology; Chronic Periodontitis - genetics; Disease Models, Animal; Etiology; Gene expression; Gene mapping; Genetic Predisposition to Disease - genetics; Genome-wide association studies; Genome-Wide Association Study; Genomes; Genotype; Gum disease; Humanities and Social Sciences; Humans; Infections; Mice; Models, Theoretical; multidisciplinary; Osteogenesis; Periodontal diseases; Periodontitis; Quantitative trait loci; Quantitative Trait Loci - genetics; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-61819-0

To suggest candidate genes involved in periodontitis, we combined gene expression data of periodontal biopsies from Collaborative Cross (CC) mouse lines, with previous reported quantitative trait loci (QTL) in mouse and with human genome-wide association studies (GWAS) associated with periodontitis. Periodontal samples from two susceptible, two resistant and two lines that showed bone formation after periodontal infection were collected during infection and naïve status. Differential expressed genes (DEGs) were analyzed in a case-control and case-only design. After infection, eleven protein-coding genes were significantly stronger expressed in resistant CC lines compared to susceptible ones. Of these, the most upregulated genes were MMP20 (P = 0.001), RSPO4 (P = 0.032), CALB1 (P = 1.06×10 −4 ), and AMTN (P = 0.05). In addition, human orthologous of candidate genes were tested for their association in a case-controls samples of aggressive (AgP) and chronic (CP) periodontitis (5,095 cases, 9,908 controls). In this analysis, variants at two loci, TTLL11/PTGS1 (rs9695213, P = 5.77×10 −5 ) and RNASE2 (rs2771342, P = 2.84×10 −5 ) suggested association with both AgP and CP. In the association analysis with AgP only, the most significant associations were located at the HLA loci HLA- DQH1 (rs9271850, P = 2.52×10 −14 ) and HLA- DPA1 (rs17214512, P = 5.14×10 −5 ). This study demonstrates the utility of the CC RIL populations as a suitable model to investigate the mechanism of periodontal disease.