Comprehensive analysis of somatic copy number alterations in clear cell renal cell carcinoma

• Published in: Molecular carcinogenesis 2020-04, Vol.59 (4), p.412-424
• Main Authors: Tsuyukubo, Takashi, Ishida, Kazuyuki, Osakabe, Mitsumasa, Shiomi, Ei, Kato, Renpei, Takata, Ryo, Obara, Wataru, Sugai, Tamotsu
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Carcinoma, Renal Cell - genetics; Carcinoma, Renal Cell - pathology; clear cell renal cell carcinoma; Clear cell-type renal cell carcinoma; Cluster Analysis; Cohort Studies; Copy number; DNA Copy Number Variations; Female; Genomes; Heterozygosity; Humans; Kaplan-Meier Estimate; Kidney cancer; Kidney Neoplasms - genetics; Kidney Neoplasms - pathology; Loss of Heterozygosity; Male; Metastases; Metastasis; Middle Aged; Multivariate Analysis; Mutation; patient prognosis; Polymorphism, Single Nucleotide; Prognosis; renal carcinogenesis; somatic copy number alteration; Statistical analysis
• ISSN: 0899-1987; 1098-2744
• DOI: 10.1002/mc.23164

Somatic copy number alterations (SCNAs) are important biological characteristics that can identify genome‐wide alterations in renal cell carcinoma (RCC). Recent studies have shown that SCNAs have potential value for determining the prognosis of RCC. We examined SCNAs using the Affymetrix platform to analyze samples from 59 patients with clear cell RCCs (ccRCCs) including first cohort (30 cases) and second cohort (validation cohort, 29 cases). We stratified SCNAs in the ccRCCs using a hierarchical cluster analysis based on SCNA types, including gain, loss of heterozygosity (LOH), copy neutral LOH, mosaic, and mixed types. In this way, the examined two cohorts were categorized into two subgroups (1 and 2). Although the frequency of mixed type was higher in subgroup 1 than in subgroup 2 in the two cohorts, the association did not reach statistical significance. There was a significant difference in the frequency of metachronous metastasis between subgroups 1 and 2 (subgroup 2 > 1). In addition, subgroup 2 was retained in multivariate analysis of both cohorts. We examined whether there were specific alleles differing between subgroups 1 and 2 in both cohorts. We found that there was indeed a statistically significant difference in the 3p mixed types. Among the 3p mixed type, we found that 3p24.3 mixed type was inversely correlated with the presence of metachronous metastasis in ccRCC. The association was also retained in multivariate analysis in second cohort. We suggest that the 3p24.3 mixed type may be a novel marker to predict a favorable prognosis in ccRCC.