An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins

Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determini...

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Bibliographic Details
Published in:Journal of biological methods 2020-02, Vol.7 (1), p.e127-e127
Main Authors: Roberts, Michael D, Young, Kaelin C, Fox, Carlton D, Vann, Christopher G, Roberson, Paul A, Osburn, Shelby C, Moore, Johnathon H, Mumford, Petey W, Romero, Matthew A, Beck, Darren T, Haun, Cody T, Badisa, Veera L D, Mwashote, Benjamin M, Ibeanusi, Victor, Kavazis, Andreas N
Format: Article
Language:English
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Summary:Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determining which method was most suitable for MF protein isolation and solubilization. Here, we provide an optimized protocol to isolate sarcoplasmic and solubilized MF protein fractions from mammalian skeletal muscle suitable for several downstream assays.
ISSN:2326-9901
2326-9901
DOI:10.14440/jbm.2020.307