miR-18a Inhibitor Suppresses Leukemia Cell Proliferation by Upregulation of PTEN Expression

BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1...

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Bibliographic Details
Published in:Medical science monitor 2020-03, Vol.26, p.e921288-e921288
Main Authors: Zhang, Liping, Kong, Lingxia, Yang, Yuzhi
Format: Article
Language:English
Subjects:
Apoptosis
Cell Proliferation - drug effects
Gene Expression Regulation, Leukemic
Humans
Lab/In Vitro Research
Leukemia - drug therapy
Leukemia - pathology
MicroRNAs - antagonists & inhibitors
PTEN Phosphohydrolase - metabolism
THP-1 Cells
Transfection
Up-Regulation
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Summary:BACKGROUND Leukemia is common in aging adults and has very high mortality worldwide. The present study was designed to investigate the therapeutic efficacy of miR-18a inhibitor against WEHI-3 and THP-1 leukemia cells. MATERIAL AND METHODS The changes in miR-18a inhibitor-transfected WEHI-3 and THP-1 cell proliferative potential was measured by use of the Cell Counting Kit-8 assay. Apoptotic changes were analyzed by electron microscopy, and evaluation of PI3K, AKT, mTOR, and PTEN expression was assessed by RT-qPCR assay. RESULTS Transfection of miR-18a inhibitor significantly (P
ISSN:1643-3750
1234-1010
1643-3750
DOI:10.12659/MSM.921288