Effect of intranasal instillation of Escherichia coli on apoptosis of spleen cells in diet-induced-obese mice

• Published in: Scientific reports 2020-03, Vol.10 (1), p.5109
• Main Authors: Ren, Zhihua, Gu, Xuchu, Fang, Jing, Cai, Dongjie, Zuo, Zhicai, Liang, Shuang, Cui, Hengmin, Deng, Junliang, Ma, Xiaoping, Geng, Yi, Zhang, Ming, Xie, Yue, Ye, Gang, Gou, Liping, Hu, Yanchun
• Format: Article
• Language: English
• Subjects: Administration, Intranasal; Animals; Apoptosis; Apoptosis - immunology; BAX protein; Bcl-2 protein; bcl-2-Associated X Protein - biosynthesis; Caspase 3 - biosynthesis; Caspase 9 - biosynthesis; Caspase-3; Caspase-9; Diet; E coli; Escherichia coli; Escherichia coli - immunology; Escherichia coli Infections - immunology; Escherichia coli Infections - microbiology; Escherichia coli Infections - mortality; Histology; Immune response; Immune system; Macrophages - immunology; Male; Megakaryocytes - immunology; Mice; Mice, Obese; Neutrophils - immunology; Obesity - immunology; Pneumonia - immunology; Pneumonia - microbiology; Pneumonia - mortality; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Spleen; Spleen - cytology; Spleen - immunology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-62044-5

Splenic immune function was enhanced in diet-induced-obese (DIO) mice caused by Escherichia coli. The changes in spleen function on apoptosis were still unknown. Two hundred mice in groups Lean-E. coli and DIO-E. coli were intranasal instillation of E. coli. And another two hundred mice in groups Lean-PBS and DIO-PBS were given phosphate-buffered saline (PBS). Subsequently, spleen histology was analyzed. Then the rates of spleen cell (SC) apoptosis, and expression of the genes and proteins of Bcl-2, Bax, caspase-3 and caspase-9 were quantified in each group at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. The SC apoptosis rates of the DIO-E. coli groups were lower than those of the DIO-PBS groups at 12, 24 and 72 h (p