Identification of heat-dissociable RNA complexes in two porcine coronaviruses

THE coronavirus genome has been shown to comprise single-stranded RNA 1,2 . Examination of the viral nucleic acid synthesised by pig kidney cells infected with transmissible gastroenteritis virus (TGEV) suggested that several molecular species, ranging in size between 18 and 28S, were involved in th...

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Bibliographic Details
Published in:Nature (London) 1975-10, Vol.257 (5526), p.508-510
Main Authors: GARWES, D. J, POCOCK, D. H, WIJASZKA, T. M
Format: Article
Language:English
Subjects:
Animals
Coronaviridae - analysis
Hot Temperature
Humanities and Social Sciences
letter
Molecular Weight
multidisciplinary
RNA, Viral - analysis
Science
Science (multidisciplinary)
Swine - microbiology
Transmissible gastroenteritis virus - analysis
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Summary:THE coronavirus genome has been shown to comprise single-stranded RNA 1,2 . Examination of the viral nucleic acid synthesised by pig kidney cells infected with transmissible gastroenteritis virus (TGEV) suggested that several molecular species, ranging in size between 18 and 28S, were involved in the viral replicative cycle 3 ; similarly Tannock found a wide variation in the size of RNA molecules extracted from avian infectious bronchitis virus (IBV) by a phenol–sodium dodecyl sulphate (SDS) method 2 . Extraction of IBV RNA by 1% SDS at 60 °C has, however, revealed a single component of molecular weight 9×10 6 corresponding to 60S by electrophoresis through 2.2% polyacrylamide gels 4 .
ISSN:0028-0836
1476-4687
DOI:10.1038/257508a0