Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody

Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to ne...

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Bibliographic Details
Published in:Archives of virology 1987-03, Vol.97 (1-2), p.123-130
Main Authors: Lecomte, J., Cainelli-Gebara, V., Mercier, G., Mansour, S., Talbot, P. J., Lussier, G., Oth, D.
Format: Article
Language:English
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Brief Report
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Summary:Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immunofluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01310740