Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs

In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a h...

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Bibliographic Details
Published in:Archives of virology 2012-01, Vol.157 (1), p.171-175
Main Authors: Ntafis, Vasileios, Xylouri, Eftychia, Mari, Viviana, Papanastassopoulou, Maria, Papaioannou, Nikolaos, Thomas, Angelos, Buonavoglia, Canio, Decaro, Nicola
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brief Report
Canine coronavirus
Coronavirus Infections - veterinary
Coronavirus Infections - virology
Coronavirus, Canine - classification
Coronavirus, Canine - genetics
Coronavirus, Canine - isolation & purification
Coronaviruses
Diarrhea - veterinary
Diarrhea - virology
Dog Diseases - virology
Dogs
Feces - virology
Fundamental and applied biological sciences. Psychology
Genomes
Infections
Infectious Diseases
Intestines - virology
Lungs
Male
Medical Microbiology
Microbiology
Miscellaneous
Molecular Sequence Data
Open Reading Frames
Phylogenetics
Phylogeny
Proteins
RNA polymerase
Spleen
Veterinary medicine
Virology
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Summary:In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-011-1141-6