Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA

In this study feline (FECV and FIPV) and canine (CCoV) coronavirus entry into and release from polarized porcine epithelial LLC-PK1 cells, stably expressing the recombinant feline aminopeptidase-N cDNA, were investigated. Virus entry appeared to occur preferentially through the apical membrane, simi...

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Bibliographic Details
Published in:Archives of virology 2001-01, Vol.146 (4), p.791-799
Main Authors: ROSSEN, J. W. A, KOUAME, J, GOEDHEER, A. J. W, VENNEMA, H, ROTTIER, P. J. M
Format: Article
Language:English
Subjects:
aminopeptidase N
Aminopeptidases - genetics
Aminopeptidases - metabolism
Animal viral diseases
Animals
Biological and medical sciences
Brief Report
Canine coronavirus
Cats - virology
Cell Polarity
Coronavirus - metabolism
Coronavirus - pathogenicity
Coronavirus, Canine - metabolism
Coronavirus, Canine - pathogenicity
DNA, Complementary
Epithelial Cells - virology
Feline coronavirus
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Infectious diseases
LLC-PK1 Cells
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Medical sciences
Microbiology
Recombinant Proteins - metabolism
Swine
Transfection
Viral diseases
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Summary:In this study feline (FECV and FIPV) and canine (CCoV) coronavirus entry into and release from polarized porcine epithelial LLC-PK1 cells, stably expressing the recombinant feline aminopeptidase-N cDNA, were investigated. Virus entry appeared to occur preferentially through the apical membrane, similar to the entry of the related porcine coronavirus transmissible gastroenteritis virus (TGEV) into these cells. However, whereas TGEV is released apically, feline and canine coronaviruses were found to be released from the basolateral side of the epithelial cells. These observations indicate that local infections as caused by TGEV, FECV and CCoV do not strictly correlate with apical release, as suggested by earlier work.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050170147