Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro

This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV r...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2009-06, Vol.154 (6), p.999-1003
Main Authors: Wei, Zhan-Yong, Wang, Xue-Bin, Ning, Xiao-Dong, Wang, Ya-Bin, Zhang, Hong-Ying, Wang, Dong-Fang, Chen, Hong-Ying, Cui, Bao-An
Format: Article
Language:English
Subjects:
Animals
Antiviral Agents - pharmacology
Arginine - pharmacology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brief Report
Cell Line
DNA, Viral - biosynthesis
Fundamental and applied biological sciences. Psychology
Hogs
Infections
Infectious Diseases
Medical Microbiology
Microbiology
Miscellaneous
Nitric oxide
Nitric Oxide - pharmacology
Parvovirus, Porcine - drug effects
Parvovirus, Porcine - physiology
Porcine parvovirus
S-Nitroso-N-Acetylpenicillamine - pharmacology
Swine
Veterinary medicine
Viral infections
Viral Proteins - biosynthesis
Virology
Virus Replication - drug effects
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-009-0392-y