Comparison of six methods for detecting human rotavirus in stools

The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 c...

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Bibliographic Details
Published in:European Journal of Clinical Microbiology 1984-04, Vol.3 (2), p.136-140
Main Authors: Morinet, F, Ferchal, F, Colimon, R, Pérol, Y
Format: Article
Language:English
Subjects:
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Feces - microbiology
Fluorescent Antibody Technique
Humans
Infant
Latex Fixation Tests
Microscopy, Electron
Original
Rotavirus - isolation & purification
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Summary:The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex , 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost , 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex .
ISSN:0722-2211
1435-4373
DOI:10.1007/BF02014331