A tandem active site model for the ribosomal helicase

During protein synthesis, the messenger RNA (mRNA) helicase activity of the ribosome ensures that codons are made single stranded before decoding. Here, based on recent structural and functional findings, a quantitative model is presented for a tandem arrangement of two helicase active sites on the...

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Published in:FEBS letters 2019-05, Vol.593 (10), p.1009-1019
Main Authors: Amiri, Hossein, Noller, Harry F.
Format: Article
Language:English
Subjects:
helicase
mechanism
mRNA
ribosome
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description During protein synthesis, the messenger RNA (mRNA) helicase activity of the ribosome ensures that codons are made single stranded before decoding. Here, based on recent structural and functional findings, a quantitative model is presented for a tandem arrangement of two helicase active sites on the ribosome. A distal site encounters mRNA structures first, one elongation cycle earlier than a proximal site. Although unwinding of encountered mRNA structures past the proximal site is required for translocation, two routes exist for translocation past the distal site: sliding, which requires unwinding, and stick‐slip, which does not. The model accounts in detail for a number of findings related to the ribosomal helicase and provides a testable framework to further study mRNA unwinding.
doi_str_mv 10.1002/1873-3468.13383
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subjects helicase
mechanism
mRNA
ribosome
title A tandem active site model for the ribosomal helicase
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