Anatomy of a viral entry platform differentially functionalized by integrins α3 and α6

• Published in: Scientific reports 2020-03, Vol.10 (1), p.5356-5356, Article 5356
• Main Authors: Finke, Jérôme, Mikuličić, Snježana, Loster, Anna-Lena, Gawlitza, Alexander, Florin, Luise, Lang, Thorsten
• Format: Article
• Language: English
• Subjects: 13/51; 14; 14/19; 14/35; 631/326/596/2560; 631/57/2282; 631/80/304; 82; 82/29; Actins - metabolism; Cell Line; Cell surface; Endocytosis; Heparan sulfate; Host-Pathogen Interactions - physiology; Human papillomavirus; Human papillomavirus 16 - pathogenicity; Humanities and Social Sciences; Humans; Infections; Integrin alpha3 - genetics; Integrin alpha3 - metabolism; Integrin alpha6 - genetics; Integrin alpha6 - metabolism; Integrins; Internalization; Keratinocytes - virology; Microscopy; multidisciplinary; Papillomaviridae; Papillomavirus Infections - pathology; Papillomavirus Infections - virology; Proteins; Science; Science (multidisciplinary); Tetraspanin 24 - metabolism; Virion - metabolism; Virion - pathogenicity; Virus attachment; Virus Internalization; Viruses
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-62202-9

During cell invasion, human papillomaviruses use large CD151 patches on the cell surface. Here, we studied whether these patches are defined architectures with features for virus binding and/or internalization. Super-resolution microscopy reveals that the patches are assemblies of closely associated nanoclusters of CD151, integrin α3 and integrin α6. Integrin α6 is required for virus attachment and integrin α3 for endocytosis. We propose that CD151 organizes viral entry platforms with different types of integrin clusters for different functionalities. Since numerous viruses use tetraspanin patches, we speculate that this building principle is a blueprint for cell-surface architectures utilized by viral particles.