Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome-associated coronavirus

SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2006-06, Vol.580 (15), p.3643-3648
Main Authors: Law, Pui Ying Peggy, Liu, Yuet-Man, Geng, Hua, Kwan, Ka Ho, Waye, Mary Miu-Yee, Ho, Yuan-Yuan
Format: Article
Language:English
Subjects:
Animals
Cell Line
Chlorocebus aethiops
Coronavirus
Cricetinae
DNA - biosynthesis
Gene Expression
Gene Expression Regulation, Viral
Genes, Reporter - genetics
SARS 8b
SARS coronavirus
SARS ORF 8
SARS Virus - genetics
SARS Virus - metabolism
Severe acute respiratory syndrome
Short Communication
Short Communications
Thymidine - genetics
Viral Proteins - genetics
Viral Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in both nucleus and cytoplasm of mammalian cells. Functional study revealed that overexpression of SARS 8b induced DNA synthesis. Coexpression of SARS 8b and SARS 6, a previously characterized SARS-CoV accessory protein, did not elicit synergistic effects on DNA synthesis.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.05.051