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Polymerase chain reaction–based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion
Objectives: To study the association of human rhinovirus (HRV), respiratory syncytial virus (RSV), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (OME). To determine how long HRV RNA can be detected after HRV infection. Methods: Middle ear effu...
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| Published in: | The Journal of pediatrics 1998-09, Vol.133 (3), p.390-394 |
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| container_title | The Journal of pediatrics |
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| creator | Pitkäranta, Anne Jero, Jussi Arruda, Eurico Virolainen, Anni Hayden, Frederick G. |
| description | Objectives: To study the association of human rhinovirus (HRV), respiratory syncytial virus (RSV), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (OME). To determine how long HRV RNA can be detected after HRV infection.
Methods: Middle ear effusion (MEE) samples collected at the time of tympanostomy tube placement from 100 children with OME were examined. Viral RNA was detected by reverse-transcriptase polymerase chain reaction. For HRV the results were compared with virus isolation in cell culture. In vitro studies of the persistence of HRV infectivity and RNA were conducted by combining ~10
5 median cell culture infectious doses of HRV with pooled MEE at 37°C and assaying serial samples for 12 weeks.
Results: Virus RNA was detected in 30 children. HRV was detected by reverse-transcriptase polymerase chain reaction in 19 children with OME and by virus isolation in 5 children. RSV RNA was found in 8 and HCV in 3 children with OME. No dual viral infection was found. Bacterial pathogens were isolated from 35 MEE samples and were associated with viral RNA in 11 cases, most often with HRV (9 cases). Under in vitro conditions, HRV culture positivity declined rapidly ( |
| doi_str_mv | 10.1016/S0022-3476(98)70276-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7095025</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022347698702768</els_id><sourcerecordid>73901785</sourcerecordid><originalsourceid>FETCH-LOGICAL-c541t-7bf03e880a943bf224c332430a34c2663bb5498cb7f516a10eca3fa9eac9b42c3</originalsourceid><addsrcrecordid>eNqFkcuKFDEUhoMoYzv6CAPBhShYenKpSrIZkcEbDCio65BKp-wM1UmbpFpq58Yn8A19EtMXGnXjKnD-__w553wIXRB4RoB0zz8CUNowLrrHSj4RQEXXyFtoQUCJppOM3UaLk-UuupfzDQAoDnCGzpRgUlC2QD8-xHFeu2Syw3ZlfMDJGVt8DL--_-xrdYmXrrh9BccBp5UPcevTlJ9WZ974ZEpMM85zsHPxZsRH0YQltjHFYPYFXJNj8cVnvHZLb_A3X1bYDcOUa_J9dGcwY3YPju85-vz61aert831-zfvrl5eN7blpDSiH4A5KcEozvqBUm4Zo5yBYdzSrmN933IlbS-GlnSGgLOGDUbVjVTPqWXn6PKQu5n6OoZ1oSQz6k3ya5NmHY3XfyvBr_SXuNUCVAu0rQGPjgEpfp1cLnrts3XjaIKLU9aCKSBC7owP_zHexCmFupwmigtOK4pqag8mm2LOyQ2nSQjoHWS9h6x3BLWSeg9Zy9p38ecap64j1aq_OOiu3nLrXdLZehdsPXyqKPUy-v_88BsXKbww</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>194742009</pqid></control><display><type>article</type><title>Polymerase chain reaction–based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion</title><source>ScienceDirect Journals</source><creator>Pitkäranta, Anne ; Jero, Jussi ; Arruda, Eurico ; Virolainen, Anni ; Hayden, Frederick G.</creator><creatorcontrib>Pitkäranta, Anne ; Jero, Jussi ; Arruda, Eurico ; Virolainen, Anni ; Hayden, Frederick G.</creatorcontrib><description>Objectives: To study the association of human rhinovirus (HRV), respiratory syncytial virus (RSV), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (OME). To determine how long HRV RNA can be detected after HRV infection.
Methods: Middle ear effusion (MEE) samples collected at the time of tympanostomy tube placement from 100 children with OME were examined. Viral RNA was detected by reverse-transcriptase polymerase chain reaction. For HRV the results were compared with virus isolation in cell culture. In vitro studies of the persistence of HRV infectivity and RNA were conducted by combining ~10
5 median cell culture infectious doses of HRV with pooled MEE at 37°C and assaying serial samples for 12 weeks.
Results: Virus RNA was detected in 30 children. HRV was detected by reverse-transcriptase polymerase chain reaction in 19 children with OME and by virus isolation in 5 children. RSV RNA was found in 8 and HCV in 3 children with OME. No dual viral infection was found. Bacterial pathogens were isolated from 35 MEE samples and were associated with viral RNA in 11 cases, most often with HRV (9 cases). Under in vitro conditions, HRV culture positivity declined rapidly (<2 days), but RNA was detectable for up to 8 weeks.
Conclusions: These results suggest that virus infection, particularly HRV infection, either alone or concurrent with bacteria, is present in a larger percentage of children with OME than previously suspected. It remains to be determined how often the presence of viral RNA in MEE represents persistent RNA, ongoing viral replication, or recurrent infection. (J Pediatr 1998;133:390-4)</description><identifier>ISSN: 0022-3476</identifier><identifier>EISSN: 1097-6833</identifier><identifier>DOI: 10.1016/S0022-3476(98)70276-8</identifier><identifier>PMID: 9738723</identifier><identifier>CODEN: JOPDAB</identifier><language>eng</language><publisher>United States: Mosby, Inc</publisher><subject>Bacterial Infections - complications ; Cells, Cultured ; Child ; Child, Preschool ; Coronavirus - genetics ; Coronavirus - isolation & purification ; Coronavirus Infections - complications ; Coronavirus Infections - diagnosis ; Female ; Humans ; Infant ; Male ; Middle Ear Ventilation ; Otitis Media with Effusion - microbiology ; Otitis Media with Effusion - virology ; Picornaviridae Infections - complications ; Picornaviridae Infections - diagnosis ; Polymerase Chain Reaction ; Recurrence ; Respiratory Syncytial Virus Infections - complications ; Respiratory Syncytial Virus Infections - diagnosis ; Respiratory Syncytial Viruses - genetics ; Respiratory Syncytial Viruses - isolation & purification ; Rhinovirus - genetics ; Rhinovirus - isolation & purification ; RNA, Viral - analysis ; RNA, Viral - genetics ; Virulence ; Virus Replication</subject><ispartof>The Journal of pediatrics, 1998-09, Vol.133 (3), p.390-394</ispartof><rights>1998 Mosby, Inc.</rights><rights>Copyright Mosby-Year Book, Inc. Sep 1998</rights><rights>Copyright © 1998 Mosby, Inc. All rights reserved. 1998 Mosby, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-7bf03e880a943bf224c332430a34c2663bb5498cb7f516a10eca3fa9eac9b42c3</citedby><cites>FETCH-LOGICAL-c541t-7bf03e880a943bf224c332430a34c2663bb5498cb7f516a10eca3fa9eac9b42c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9738723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pitkäranta, Anne</creatorcontrib><creatorcontrib>Jero, Jussi</creatorcontrib><creatorcontrib>Arruda, Eurico</creatorcontrib><creatorcontrib>Virolainen, Anni</creatorcontrib><creatorcontrib>Hayden, Frederick G.</creatorcontrib><title>Polymerase chain reaction–based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion</title><title>The Journal of pediatrics</title><addtitle>J Pediatr</addtitle><description>Objectives: To study the association of human rhinovirus (HRV), respiratory syncytial virus (RSV), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (OME). To determine how long HRV RNA can be detected after HRV infection.
Methods: Middle ear effusion (MEE) samples collected at the time of tympanostomy tube placement from 100 children with OME were examined. Viral RNA was detected by reverse-transcriptase polymerase chain reaction. For HRV the results were compared with virus isolation in cell culture. In vitro studies of the persistence of HRV infectivity and RNA were conducted by combining ~10
5 median cell culture infectious doses of HRV with pooled MEE at 37°C and assaying serial samples for 12 weeks.
Results: Virus RNA was detected in 30 children. HRV was detected by reverse-transcriptase polymerase chain reaction in 19 children with OME and by virus isolation in 5 children. RSV RNA was found in 8 and HCV in 3 children with OME. No dual viral infection was found. Bacterial pathogens were isolated from 35 MEE samples and were associated with viral RNA in 11 cases, most often with HRV (9 cases). Under in vitro conditions, HRV culture positivity declined rapidly (<2 days), but RNA was detectable for up to 8 weeks.
Conclusions: These results suggest that virus infection, particularly HRV infection, either alone or concurrent with bacteria, is present in a larger percentage of children with OME than previously suspected. It remains to be determined how often the presence of viral RNA in MEE represents persistent RNA, ongoing viral replication, or recurrent infection. (J Pediatr 1998;133:390-4)</description><subject>Bacterial Infections - complications</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Coronavirus - genetics</subject><subject>Coronavirus - isolation & purification</subject><subject>Coronavirus Infections - complications</subject><subject>Coronavirus Infections - diagnosis</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Middle Ear Ventilation</subject><subject>Otitis Media with Effusion - microbiology</subject><subject>Otitis Media with Effusion - virology</subject><subject>Picornaviridae Infections - complications</subject><subject>Picornaviridae Infections - diagnosis</subject><subject>Polymerase Chain Reaction</subject><subject>Recurrence</subject><subject>Respiratory Syncytial Virus Infections - complications</subject><subject>Respiratory Syncytial Virus Infections - diagnosis</subject><subject>Respiratory Syncytial Viruses - genetics</subject><subject>Respiratory Syncytial Viruses - isolation & purification</subject><subject>Rhinovirus - genetics</subject><subject>Rhinovirus - isolation & purification</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - genetics</subject><subject>Virulence</subject><subject>Virus Replication</subject><issn>0022-3476</issn><issn>1097-6833</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkcuKFDEUhoMoYzv6CAPBhShYenKpSrIZkcEbDCio65BKp-wM1UmbpFpq58Yn8A19EtMXGnXjKnD-__w553wIXRB4RoB0zz8CUNowLrrHSj4RQEXXyFtoQUCJppOM3UaLk-UuupfzDQAoDnCGzpRgUlC2QD8-xHFeu2Syw3ZlfMDJGVt8DL--_-xrdYmXrrh9BccBp5UPcevTlJ9WZ974ZEpMM85zsHPxZsRH0YQltjHFYPYFXJNj8cVnvHZLb_A3X1bYDcOUa_J9dGcwY3YPju85-vz61aert831-zfvrl5eN7blpDSiH4A5KcEozvqBUm4Zo5yBYdzSrmN933IlbS-GlnSGgLOGDUbVjVTPqWXn6PKQu5n6OoZ1oSQz6k3ya5NmHY3XfyvBr_SXuNUCVAu0rQGPjgEpfp1cLnrts3XjaIKLU9aCKSBC7owP_zHexCmFupwmigtOK4pqag8mm2LOyQ2nSQjoHWS9h6x3BLWSeg9Zy9p38ecap64j1aq_OOiu3nLrXdLZehdsPXyqKPUy-v_88BsXKbww</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Pitkäranta, Anne</creator><creator>Jero, Jussi</creator><creator>Arruda, Eurico</creator><creator>Virolainen, Anni</creator><creator>Hayden, Frederick G.</creator><general>Mosby, Inc</general><general>Mosby-Year Book, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>U9A</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980901</creationdate><title>Polymerase chain reaction–based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion</title><author>Pitkäranta, Anne ; Jero, Jussi ; Arruda, Eurico ; Virolainen, Anni ; Hayden, Frederick G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-7bf03e880a943bf224c332430a34c2663bb5498cb7f516a10eca3fa9eac9b42c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bacterial Infections - complications</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Coronavirus - genetics</topic><topic>Coronavirus - isolation & purification</topic><topic>Coronavirus Infections - complications</topic><topic>Coronavirus Infections - diagnosis</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Middle Ear Ventilation</topic><topic>Otitis Media with Effusion - microbiology</topic><topic>Otitis Media with Effusion - virology</topic><topic>Picornaviridae Infections - complications</topic><topic>Picornaviridae Infections - diagnosis</topic><topic>Polymerase Chain Reaction</topic><topic>Recurrence</topic><topic>Respiratory Syncytial Virus Infections - complications</topic><topic>Respiratory Syncytial Virus Infections - diagnosis</topic><topic>Respiratory Syncytial Viruses - genetics</topic><topic>Respiratory Syncytial Viruses - isolation & purification</topic><topic>Rhinovirus - genetics</topic><topic>Rhinovirus - isolation & purification</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - genetics</topic><topic>Virulence</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pitkäranta, Anne</creatorcontrib><creatorcontrib>Jero, Jussi</creatorcontrib><creatorcontrib>Arruda, Eurico</creatorcontrib><creatorcontrib>Virolainen, Anni</creatorcontrib><creatorcontrib>Hayden, Frederick G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of pediatrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pitkäranta, Anne</au><au>Jero, Jussi</au><au>Arruda, Eurico</au><au>Virolainen, Anni</au><au>Hayden, Frederick G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase chain reaction–based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion</atitle><jtitle>The Journal of pediatrics</jtitle><addtitle>J Pediatr</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>133</volume><issue>3</issue><spage>390</spage><epage>394</epage><pages>390-394</pages><issn>0022-3476</issn><eissn>1097-6833</eissn><coden>JOPDAB</coden><abstract>Objectives: To study the association of human rhinovirus (HRV), respiratory syncytial virus (RSV), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (OME). To determine how long HRV RNA can be detected after HRV infection.
Methods: Middle ear effusion (MEE) samples collected at the time of tympanostomy tube placement from 100 children with OME were examined. Viral RNA was detected by reverse-transcriptase polymerase chain reaction. For HRV the results were compared with virus isolation in cell culture. In vitro studies of the persistence of HRV infectivity and RNA were conducted by combining ~10
5 median cell culture infectious doses of HRV with pooled MEE at 37°C and assaying serial samples for 12 weeks.
Results: Virus RNA was detected in 30 children. HRV was detected by reverse-transcriptase polymerase chain reaction in 19 children with OME and by virus isolation in 5 children. RSV RNA was found in 8 and HCV in 3 children with OME. No dual viral infection was found. Bacterial pathogens were isolated from 35 MEE samples and were associated with viral RNA in 11 cases, most often with HRV (9 cases). Under in vitro conditions, HRV culture positivity declined rapidly (<2 days), but RNA was detectable for up to 8 weeks.
Conclusions: These results suggest that virus infection, particularly HRV infection, either alone or concurrent with bacteria, is present in a larger percentage of children with OME than previously suspected. It remains to be determined how often the presence of viral RNA in MEE represents persistent RNA, ongoing viral replication, or recurrent infection. (J Pediatr 1998;133:390-4)</abstract><cop>United States</cop><pub>Mosby, Inc</pub><pmid>9738723</pmid><doi>10.1016/S0022-3476(98)70276-8</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of pediatrics, 1998-09, Vol.133 (3), p.390-394 |
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| language | eng |
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| source | ScienceDirect Journals |
| subjects | Bacterial Infections - complications Cells, Cultured Child Child, Preschool Coronavirus - genetics Coronavirus - isolation & purification Coronavirus Infections - complications Coronavirus Infections - diagnosis Female Humans Infant Male Middle Ear Ventilation Otitis Media with Effusion - microbiology Otitis Media with Effusion - virology Picornaviridae Infections - complications Picornaviridae Infections - diagnosis Polymerase Chain Reaction Recurrence Respiratory Syncytial Virus Infections - complications Respiratory Syncytial Virus Infections - diagnosis Respiratory Syncytial Viruses - genetics Respiratory Syncytial Viruses - isolation & purification Rhinovirus - genetics Rhinovirus - isolation & purification RNA, Viral - analysis RNA, Viral - genetics Virulence Virus Replication |
| title | Polymerase chain reaction–based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion |
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