Direct Blood Culturing of Candida spp. on Solid Medium by a Rapid Enrichment Method with Magnetic Beads Coated with Recombinant Human Mannan-Binding Lectin

A rapid and accurate method to identify the species and antibiotic resistance of spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of spp. in blood make rapid diagnosis by standard blood culture difficult. In this study,...

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Bibliographic Details
Published in:Journal of clinical microbiology 2020-03, Vol.58 (4)
Main Authors: Chen, Xiao-Li, Zheng, Hao, Li, Wen-Ge, Zhong, You-Hong, Chen, Xiao-Ping, Lu, Jin-Xing
Format: Article
Language:English
Subjects:
Mycology
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Summary:A rapid and accurate method to identify the species and antibiotic resistance of spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we constructed a direct blood culturing method (i.e., the M1 method) by a rapid enrichment method with magnetic beads coated with a recombined human mannan-binding lectin (rhMBL; i.e., M1 protein), which demonstrated much higher sp.-binding capacity than that of full-length MBL expressed (i.e., M2). With the M1 method, individual colonies were obtained before the standard blood culture method for each species of spp. tested at 
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00057-20