In vitro hepatic aflatoxicol production is related to a higher resistance to aflatoxin B1 in poultry

A study was conducted to determine the cytosolic in vitro hepatic enzymatic kinetic parameters V m a x , K M , and intrinsic clearance (CL i n t ) for aflatoxin B 1 (AFB 1 ) reductase [aflatoxicol (AFL) production] and AFL dehydrogenase (AFB 1 production) in four commercial poultry species (chicken,...

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Bibliographic Details
Published in:Scientific reports 2020-03, Vol.10 (1), Article 5508
Main Authors: Murcia, Hansen W., Diaz, Gonzalo J.
Format: Article
Language:English
Subjects:
631/45/607/1168
631/92/607/1168
Aflatoxin B1
Aflatoxins
Aquatic birds
Chickens
Dehydrogenase
Dehydrogenases
Ducks
Enzymatic activity
Humanities and Social Sciences
Liver
Metabolites
multidisciplinary
Oxidation
Poultry
Reductase
Science
Science (multidisciplinary)
Species
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Summary:A study was conducted to determine the cytosolic in vitro hepatic enzymatic kinetic parameters V m a x , K M , and intrinsic clearance (CL i n t ) for aflatoxin B 1 (AFB 1 ) reductase [aflatoxicol (AFL) production] and AFL dehydrogenase (AFB 1 production) in four commercial poultry species (chicken, quail, turkey and duck). Large differences were found in AFB 1 reductase activity, being the chicken the most efficient producer of AFL (highest CL i n t value). Oxidation of AFL to AFB 1 showed only slight differences among the different poultry species. On average all species produced AFB 1 from AFL at a similar rate, except for the turkey which produced AFB 1 from AFL at a significantly lower rate than chickens and quail, but not ducks. Although the turkey and duck showed differences in AFL oxidation V m a x and K M parameters, their CL i n t values did not differ significantly. The ratio AFB 1 reductase/AFL dehydrogenase enzyme activity was inversely related to the known in vivo sensitivity to AFB 1 being highest for the chicken, lowest for the duck and intermediate for turkeys and quail. Since there is no evidence that AFL is a toxic metabolite of AFB 1 , these results suggest that AFL production is a detoxication reaction in poultry. Conversion of AFB 1 to AFL prevents the formation of the AFB 1 -8,9- exo -epoxide which, upon conversion to AFB 1 -dihydrodiol, is considered to be the metabolite responsible for the acute toxic effects of AFB 1 .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-62415-y