Stem cell differentiation trajectories in Hydra resolved at single-cell resolution

The adult polyp continually renews all of its cells using three separate stem cell populations, but the genetic pathways enabling this homeostatic tissue maintenance are not well understood. We sequenced 24,985 single-cell transcriptomes and identified the molecular signatures of a broad spectrum of...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2019-07, Vol.365 (6451)
Main Authors: Siebert, Stefan, Farrell, Jeffrey A, Cazet, Jack F, Abeykoon, Yashodara, Primack, Abby S, Schnitzler, Christine E, Juliano, Celina E
Format: Article
Language:English
Subjects:
Anatomy
Animals
Biomarkers
Cascades
Cell differentiation
Cell Differentiation - genetics
Cell fate
Cell lineage
Cell Lineage - genetics
Cell self-renewal
Construction
Developmental stages
Differentiation (biology)
Ectoderm
Endoderm
Epithelial cells
Extracellular matrix
Flow cytometry
Fluorescence
Functional testing
Gene expression
Gene mapping
Gene sequencing
Genes
Genetics
Hybridization
Hydra - cytology
Hydra - genetics
Hydra - growth & development
Individualized Instruction
Mapping
Modules
Nervous system
Neural stem cells
Neurogenesis
Neurons
Neurosciences
Pattern formation
Population genetics
Populations
Questions
Regeneration
Regulators
Regulatory sequences
Ribonucleic acid
RNA
Signatures
Single-Cell Analysis
Specifications
State regulations
Stem cell transplantation
Stem cells
Stem Cells - cytology
Trajectories
Transcription factors
Transcriptome
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Summary:The adult polyp continually renews all of its cells using three separate stem cell populations, but the genetic pathways enabling this homeostatic tissue maintenance are not well understood. We sequenced 24,985 single-cell transcriptomes and identified the molecular signatures of a broad spectrum of cell states, from stem cells to terminally differentiated cells. We constructed differentiation trajectories for each cell lineage and identified gene modules and putative regulators expressed along these trajectories, thus creating a comprehensive molecular map of all developmental lineages in the adult animal. In addition, we built a gene expression map of the nervous system. Our work constitutes a resource for addressing questions regarding the evolution of metazoan developmental processes and nervous system function.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aav9314