Non-culture based assays for the detection of fungal pathogens

Traditional, culture based methods for the diagnosis of fungal infections are still considered as gold standard, but they are time consuming and low sensitive. Therefore, in order to overcome the limitations, many researchers have focused on the development of new immunological and molecular based r...

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Bibliographic Details
Published in:Journal de mycologie médicale 2018-06, Vol.28 (2), p.236-248
Main Authors: Otašević, S., Momčilović, S., Stojanović, N.M., Skvarč, M., Rajković, K., Arsić-Arsenijević, V.
Format: Article
Language:English
Subjects:
Biological Assay
Conventional assays
Dermatomycoses - diagnosis
Fungal infection
Fungi - isolation & purification
Fungi - pathogenicity
Humans
Immunoassay
Immunoassays
Immunologic Tests
Invasive Fungal Infections - diagnosis
Molecular assays
Molecular Diagnostic Techniques - methods
Mycoses - diagnosis
Polymerase Chain Reaction
Rapid detection
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Summary:Traditional, culture based methods for the diagnosis of fungal infections are still considered as gold standard, but they are time consuming and low sensitive. Therefore, in order to overcome the limitations, many researchers have focused on the development of new immunological and molecular based rapid assays that could enable early diagnosis of infection and accurate identification of fungal pathogens causing superficial and invasive infection. In this brief review, we highlighted the advantages and disadvantages of conventional diagnostic methods and possibility of non-culture based assays in diagnosis of superficial fungal infections and presented the overview on currently available immunochromatographic assays as well as availability of biomarkers detection by immunodiagnostic procedures in prompt and accurate diagnosis of invasive fungal infections. In addition, we presented diagnostic efficiency of currently available molecular panels and researches in this area.
ISSN:1156-5233
1773-0449
DOI:10.1016/j.mycmed.2018.03.001