Detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from Brazil

•Feline vesivirus RNA was detected and sequenced in seven samples from diarrheic cats.•One sample was characterized as a feline norovirus (FeNoV) by phylogenetic analysis.•Norovirus genogroup IV (GIV) detection and quantification was performed using quantitative one step RT-PCR.•FeNoV GIV was detect...

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Bibliographic Details
Published in:The veterinary journal (1997) 2015-10, Vol.206 (1), p.115-117
Main Authors: Castro, Tatiana X., Cubel Garcia, Rita de Cássia N., Fumian, Tulio M., Costa, Erika M., Mello, Renata, White, Peter A., Leite, José Paulo G.
Format: Article
Language:English
Subjects:
Animals
Brazil
Caliciviridae Infections - epidemiology
Caliciviridae Infections - veterinary
Caliciviridae Infections - virology
Calicivirus
Cat
Cat Diseases - epidemiology
Cat Diseases - virology
Cats
Disease Outbreaks - veterinary
Gastroenteritis - epidemiology
Gastroenteritis - veterinary
Gastroenteritis - virology
Norovirus
Norovirus - genetics
Norovirus - isolation & purification
Phylogeny
Real-time PCR
Sequence analysis
Vesivirus - genetics
Vesivirus - isolation & purification
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Summary:•Feline vesivirus RNA was detected and sequenced in seven samples from diarrheic cats.•One sample was characterized as a feline norovirus (FeNoV) by phylogenetic analysis.•Norovirus genogroup IV (GIV) detection and quantification was performed using quantitative one step RT-PCR.•FeNoV GIV was detected at 5.98 × 105 genome copies per gram of stool sample.•This is the first report of enteric feline calicivirus (FCV) and FeNoV in diarrheic cats in South America. Feline caliciviruses (FCVs) have occasionally been described in cats in association with enteric disease, but an etiological role for these viruses in acute gastroenteritis is still unclear. In this study, molecular characterization of FCV and feline norovirus (FNoV) was undertaken using real-time PCR (RT-PCR) and sequence analysis of the ORF1 region in fecal specimens from 29 diarrheic cats. The specimens were also screened for parvovirus, coronavirus, astrovirus and group A rotavirus. A quantitative one step RT-PCR was also performed to detect and quantitate NoV genogroup IV and the role of these animal caliciviruses in feline gastroenteritis was investigated. This is the first description of enteric FCV and FNoV in South America.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2015.06.014