Three new coumarins from Saposhnikovia divaricata and their porcine epidemic diarrhea virus (PEDV) inhibitory activity

Swine based agro-industries throughout the world are in big threat of new PEDV infection due to lack of efficient prophylactic defenses as well as dependable curing agents. Bioactivity-guided fractionation of a methanol soluble extract from radix of Saposhnikovia divaricata led to the isolation of t...

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Bibliographic Details
Published in:Tetrahedron 2015-07, Vol.71 (28), p.4651-4658
Main Authors: Yang, Jun-Li, Dhodary, Basanta, Quy Ha, Thi Kim, Kim, Jinwoong, Kim, Eunhee, Oh, Won Keun
Format: Article
Language:English
Subjects:
agricultural industry
chemical reactions
chemical structure
Coumarin glycosides
coumarins
curing agents
dose response
fractionation
genes
methanol
nucleocapsid
PEDV inhibitory activity
Porcine epidemic diarrhea virus
protein synthesis
quantitative polymerase chain reaction
Saposhnikovia divaricata
spectroscopy
Stereochemistry
structural proteins
swine
Western blotting
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Summary:Swine based agro-industries throughout the world are in big threat of new PEDV infection due to lack of efficient prophylactic defenses as well as dependable curing agents. Bioactivity-guided fractionation of a methanol soluble extract from radix of Saposhnikovia divaricata led to the isolation of three new (1–3) together with 10 known coumarins (4–13). The structures of new isolates (1–3) were established by extensive spectroscopic analysis and their absolute configurations were assigned based on ECD spectra calculation and analysis. Among all isolates, compound 5 revealed strongest inhibitory effect on PEDV replication. Quantitative real-time PCR data showed inhibitory effect of 5 on genes responsible for synthesis of PEDV vital structural proteins (GP6 nucleocapsid, GP2 spike, and GP5 membrane) in a dose-dependent manner. Also, compound 5 demonstrated the inhibitory effect on PEDV GP6 nucleocapsid and GP2 spike protein synthesis as analyzed by western blotting. This study represents a new class of chemical entities for developing anti-PEDV agents. [Display omitted]
ISSN:0040-4020
1464-5416
0040-4020
DOI:10.1016/j.tet.2015.04.092