A new nidovirus (NamDinh virus NDiV): Its ultrastructural characterization in the C6/36 mosquito cell line

Abstract We describe the ultrastructure of the NamDinh virus (NDiV), a new member of the order Nidovirales grown in the C6/36 mosquito cell line. Uninfected and NDiV-infected cells were investigated by electron microscopy 24–48 h after infection. The results show that the viral nucleocapsid-like par...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2013-09, Vol.444 (1), p.337-342
Main Authors: Thuy, Nguyen Thanh, Huy, Tran Quang, Nga, Phan Thi, Morita, Kouichi, Dunia, Irene, Benedetti, Lucio
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
CELL CULTURES
Cell Line
Cell Membrane - virology
Culicidae
CYTOPLASM
Cytoplasm - virology
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
Immunogold
Infectious Disease
Life Sciences
MEMBRANES
Microbiology and Parasitology
Microscopy, Electron
MORPHOGENESIS
MOSQUITOES
NamDinh virus
NDiV
Nidovirales - isolation & purification
Nidovirales - physiology
Nidovirales - ultrastructure
Nidovirus
Organelles - ultrastructure
Organelles - virology
PARTICLES
RNA
SACCHAROSE
SURFACES
ULTRACENTRIFUGATION
Ultrastructure
Virion - ultrastructure
Virology
Virus Release
VIRUSES
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract We describe the ultrastructure of the NamDinh virus (NDiV), a new member of the order Nidovirales grown in the C6/36 mosquito cell line. Uninfected and NDiV-infected cells were investigated by electron microscopy 24–48 h after infection. The results show that the viral nucleocapsid-like particles form clusters concentrated in the vacuoles, the endoplasmic reticulum, and are scattered in the cytoplasm. Mature virions of NDiV were released as budding particles on the cell surface where viral components appear to lie beneath and along the plasma membrane. Free homogeneous virus particles were obtained by ultracentrifugation on sucrose gradients of culture fluids. The size of the round-shaped particles with a complete internal structure was 80 nm in diameter. This is the first study to provide information on the morphogenesis and ultrastructure of the first insect nidovirus NDiV, a missing evolutionary link in the emergence of the viruses with the largest RNA genomes.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2013.06.030