Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests

Summary Objectives A severe acute respiratory syndrome (SARS)-like disease due to a novel betacoronavirus, human coronavirus EMC (HCoV-EMC), has emerged recently. HCoV-EMC is phylogenetically closely related to Tylonycteris -bat-coronavirus-HKU4 and Pipistrellus -bat-coronavirus-HKU5 in Hong Kong. W...

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Bibliographic Details
Published in:The Journal of infection 2013-08, Vol.67 (2), p.130-140
Main Authors: Chan, Kwok-Hung, Chan, Jasper Fuk-Woo, Tse, Herman, Chen, Honglin, Lau, Candy Choi-Yi, Cai, Jian-Piao, Tsang, Alan Ka-Lun, Xiao, Xincai, To, Kelvin Kai-Wang, Lau, Susanna Kar-Pui, Woo, Patrick Chiu-Yat, Zheng, Bo-Jiang, Wang, Ming, Yuen, Kwok-Yung
Format: Article
Language:English
Subjects:
Adult
Aged
Animals
Antibodies, Neutralizing - blood
Antibodies, Viral - blood
Antibody
Betacoronavirus
Biological and medical sciences
China
Coronaviridae - immunology
Coronavirus
Cross Reactions
Cross-reactive
EMC
Female
Fluorescent Antibody Technique, Indirect - methods
General aspects
HKU1
Human viral diseases
Humans
Infectious Disease
Infectious diseases
Male
Medical sciences
Middle Aged
Neutralization
Neutralization Tests - methods
OC43
SARS
SARS coronavirus
Seroepidemiologic Studies
Seroprevalence
Severe Acute Respiratory Syndrome - immunology
Viral diseases
Viral diseases of the respiratory system and ent viral diseases
Young Adult
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Summary:Summary Objectives A severe acute respiratory syndrome (SARS)-like disease due to a novel betacoronavirus, human coronavirus EMC (HCoV-EMC), has emerged recently. HCoV-EMC is phylogenetically closely related to Tylonycteris -bat-coronavirus-HKU4 and Pipistrellus -bat-coronavirus-HKU5 in Hong Kong. We conducted a seroprevalence study on archived sera from 94 game-food animal handlers at a wild life market, 28 SARS patients, and 152 healthy blood donors in Southern China to assess the zoonotic potential and evidence for intrusion of HCoV-EMC and related viruses into humans. Methods Anti-HCoV-EMC and anti-SARS-CoV antibodies were detected using screening indirect immunofluorescence (IF) and confirmatory neutralizing antibody tests. Results Two (2.1%) animal handlers had IF antibody titer of ≥1:20 against both HCoV-EMC and SARS-CoV with neutralizing antibody titer of
ISSN:0163-4453
1532-2742
DOI:10.1016/j.jinf.2013.03.015