Tortuosity-powered microfluidic device for assessment of thrombosis and antithrombotic therapy in whole blood

Accurate assessment of blood thrombosis and antithrombotic therapy is essential for the management of patients in a variety of clinical conditions, including surgery and on extracorporeal life support. However, current monitoring devices do not measure the effects of hemodynamic forces that contribu...

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Bibliographic Details
Published in:Scientific reports 2020-04, Vol.10 (1), p.5742-5742, Article 5742
Main Authors: Luna, David J., R. Pandian, Navaneeth K., Mathur, Tanmay, Bui, Justin, Gadangi, Pranav, Kostousov, Vadim V., Hui, Shiu-Ki Rocky, Teruya, Jun, Jain, Abhishek
Format: Article
Language:English
Subjects:
639/166/985
692/699/75/593/567
Anticoagulants - pharmacology
Anticoagulants - therapeutic use
Biomimetics
Blood
Blood coagulation
Blood Coagulation - drug effects
Blood Coagulation Tests - instrumentation
Blood Platelets - cytology
Blood Platelets - drug effects
Child
Child, Preschool
Clotting
Coagulation
Drug Monitoring - instrumentation
Equipment Design
Extracorporeal Membrane Oxygenation
Fibrin
Fibrin - metabolism
Fibrinolytic Agents - pharmacology
Fibrinolytic Agents - therapeutic use
Humanities and Social Sciences
Humans
Lab-On-A-Chip Devices
Microfluidics
multidisciplinary
Oxygenation
Patients
Pediatrics
Platelets
Pressure sensors
Science
Science (multidisciplinary)
Surgery
Thromboembolism
Thrombosis
Thrombosis - blood
Thrombosis - drug therapy
Thrombosis - metabolism
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Summary:Accurate assessment of blood thrombosis and antithrombotic therapy is essential for the management of patients in a variety of clinical conditions, including surgery and on extracorporeal life support. However, current monitoring devices do not measure the effects of hemodynamic forces that contribute significantly to coagulation, platelet function and fibrin formation. This limits the extent to which current assays can predict clotting status in patients. Here, we demonstrate that a biomimetic microfluidic device consisting stenosed and tortuous arteriolar vessels would analyze blood clotting under flow, while requiring a small blood volume. When the device is connected to an inline pressure sensor a clotting time analysis is applied, allowing for the accurate measurement of coagulation, platelets and fibrin content. Furthermore, this device detects a prolonged clotting time in clinical blood samples drawn from pediatric patients on extracorporeal membrane oxygenation receiving anticoagulant therapy. Thus, this tortuosity activated microfluidic device could lead to a more quantitative and rapid assessment of clotting disorders and their treatment.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-62768-4