Combination Therapy of Wuweizi (Schisandrae Chinensis Fructus) and Dexamethasone Alleviated Dexamethasone-Induced Glucocorticoid Osteoporosis in Rats with Idiopathic Pulmonary Fibrosis

• Published in: BioMed research international 2020, Vol.2020 (2020), p.1-9
• Main Authors: Han, Yongpeng, Zhai, Huaqiang, Li, Keao, Liu, Jiao, Zhang, Nan, Gu, Min, Liu, Guoxiu, Zhang, Xiaojuan, Li, Siyu, Qin, Zinan, Ji, Zhihong
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Acid phosphatase (tartrate-resistant); Acid resistance; Alkaline phosphatase; Alveolitis; Angiogenesis; Animals; Basic Helix-Loop-Helix Transcription Factors - metabolism; Biochemical markers; Biomedical materials; Bleomycin; Bleomycin - adverse effects; Bone and Bones - pathology; Bone marrow; Bone Marrow - metabolism; Bone Marrow - pathology; Bone turnover; Bones; Calcium; Cancellous bone; Cancellous Bone - pathology; Central nervous system depressants; Chinese medicine; Combination therapy; Complications and side effects; Cytokines; Dexamethasone; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Endostatin; Endostatins - metabolism; Enzyme-linked immunosorbent assay; Epithelium; Ethylenediaminetetraacetic acid; Eye Proteins - metabolism; Fibrosis; Glucocorticoids; Glucocorticoids - therapeutic use; Growth factors; Herbal medicine; Hypoxia; Hypoxia-inducible factors; Idiopathic Pulmonary Fibrosis - complications; Idiopathic Pulmonary Fibrosis - pathology; Lung - metabolism; Lung - pathology; Lung diseases; Lungs; Male; Metabolism; Morphology; Nerve Growth Factors - metabolism; Osteoporosis; Osteoporosis - chemically induced; Osteoporosis - drug therapy; Osteoporosis - pathology; Phosphorus; Pigment epithelium-derived factor; Plant Extracts - therapeutic use; Platelet-derived growth factor; Platelet-Derived Growth Factor - metabolism; Pulmonary fibrosis; Rats; Rats, Wistar; Schisandra - chemistry; Serpins - metabolism; Steroids; Tartrate-Resistant Acid Phosphatase
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2020/6301697

Objective. To investigate the therapeutic effect of combined application of Wuweizi (Schisandrae Chinensis Fructus) and dexamethasone in rats with idiopathic pulmonary fibrosis (IPF) and the possible protective effect of Wuweizi against dexamethasone-induced glucocorticoid osteoporosis (GIOP). Methods. There were five groups in this study, including the sham operation group, model group, Wuweizi group, dexamethasone group, and the combination group. A rat IPF model was made by the endotracheal injection of bleomycin. After modeling, rats were given drug interventions for 7 and 28 days. Rats were sacrificed for pathological morphology examination of the bone and lung and quantitative determination of biochemical markers of bone metabolism and angiogenesis-related cytokine to observe therapeutic efficacy on the 7th and 28th day. ELISA was used for the quantitative determination of tartrate-resistant acid phosphatase (TRACP), bone alkaline phosphatase (BALP), hypoxia-inducible factor (HIF-1α), platelet-derived growth factor (PDGF), pigment epithelium-derived factor (PEDF), and endostatin in serum. The concentrations of calcium (Ca) and phosphorus (P) were detected with the automatic biochemical analyzer. Results. After drug interventions for 7 and 28 days, alveolitis and pulmonary fibrosis in treatment groups showed significant improvement compared with those in the model group (P