Characterization of BIV Env core: Implication for mechanism of BIV-mediated cell fusion

Entry of lentiviruses, such as human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV), requires folding of two heptad repeat regions (HR1 and HR2) of gp41 into a trimer-of-hairpins, which subsequently brings virus and cell membrane into fusion. This motif is a generalize...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-04, Vol.329 (2), p.603-609
Main Authors: Li, Shu, Zhu, Jieqing, Peng, Yu, Cui, Shanshan, Wang, Chunping, Gao, George F., Tien, Po
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding Sites
Bovine immunodeficiency virus
Cattle
Cell Fusion
Cells, Cultured
Env protein
Escherichia coli
Feline immunodeficiency virus
Heptad repeat region
Human immunodeficiency virus 1
Immunodeficiency Virus, Bovine - physiology
Lentivirus
Lung - cytology
Lung - virology
Membrane Fusion - physiology
Molecular Conformation
Molecular Sequence Data
Molecular Weight
Protein Binding
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Simian immunodeficiency virus
Six-helix bundle
Viral Envelope Proteins - metabolism
Viral Fusion Proteins - chemistry
Viral Fusion Proteins - metabolism
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Summary:Entry of lentiviruses, such as human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV), requires folding of two heptad repeat regions (HR1 and HR2) of gp41 into a trimer-of-hairpins, which subsequently brings virus and cell membrane into fusion. This motif is a generalized feature of viral fusion proteins and has been exploited in generating antiviral fusion agents. In the present paper, we report structural characters of Env protein from another lentivirus, bovine immunodeficiency virus (BIV), which contributes to a good animal model of HIV. BIV HR1 and HR2 regions are predicted by two different programs and expressed separately or conjointly in Escherichia coli. Biochemical and biophysical analyses show that the predicted HRs of BIV Env can form a stable trimer-of-hairpins or six-helix bundle just like that formed by feline immunodeficiency virus Env. Cell fusion assay demonstrates that the HR2 peptide of BIV can efficiently inhibit the virus-mediated cell fusion.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.01.160