Genetic and molecular characterization of multicomponent resistance of Pseudomonas against allicin

The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant (...

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Bibliographic Details
Published in:Life science alliance 2020-05, Vol.3 (5), p.e202000670
Main Authors: Borlinghaus, Jan, Bolger, Anthony, Schier, Christina, Vogel, Alexander, Usadel, Björn, Gruhlke, Martin Ch, Slusarenko, Alan J
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - metabolism
Disulfides - metabolism
Disulfides - pharmacology
Drug Resistance, Bacterial - genetics
Garlic - metabolism
Glutathione - metabolism
Oxidation-Reduction
Pseudomonas - genetics
Sulfinic Acids - metabolism
Sulfinic Acids - pharmacology
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Summary:The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant ( AR-1), which was isolated from garlic, conferred allicin resistance to and even to Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in AR-1, achieved through horizontal gene transfer.
ISSN:2575-1077
2575-1077
DOI:10.26508/lsa.202000670