Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction

A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tis...

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Bibliographic Details
Published in:Journal of virological methods 1997-03, Vol.64 (2), p.125-130
Main Authors: Falcone, Emiliana, D'Amore, Emanuela, Di Trani, Livia, Sili, Amalia, Tollis, Maria
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
Aves
AVIAN INFECTIOUS BRONCHITIS VIRUS
Biological and medical sciences
Chick Embryo
Chickens - virology
Chlorocebus aethiops
Coronavirus Infections - diagnosis
Coronavirus Infections - pathology
Coronavirus Infections - veterinary
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
DNA, Viral - analysis
Infectious Bronchitis Virus
Infectious bronchitis virus - genetics
Infectious bronchitis virus - isolation & purification
Infectious diseases
Medical sciences
PCR
Polymerase Chain Reaction
Polymerase Chain Reaction - veterinary
Poultry Diseases - diagnosis
Poultry Diseases - pathology
Poultry Diseases - virology
Research Paper
Vero Cells
Viral diseases
VIRUS BRONCHITE INFECTIEUSE AVIAIRE
VIRUS BRONQUITIS INFECCIOSA AVIAR
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Description
Summary:A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(96)02151-9