Controlled release of PEI/DNA complexes from mannose-bearing chitosan microspheres as a potent delivery system to enhance immune response to HBV DNA vaccine

A novel approach involving the preparation of mannose-bearing chitosan microspheres with entrapping complexes of HBV DNA and PEI was developed to improve the delivery of DNA into antigen-presenting cells (APCs) after intramuscular (i.m.) injection. Compared with the traditional chitosan microspheres...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2007-08, Vol.121 (3), p.200-207
Main Authors: Zhou, Xianfeng, Liu, Bin, Yu, Xianghui, Zha, Xiao, Zhang, Xizhen, Chen, Yu, Wang, Xueyun, Jin, Yinghua, Wu, Yongge, Chen, Yue, Shan, Yaming, Chen, Yan, Liu, Junqiu, Kong, Wei, Shen, Jiacong
Format: Article
Language:English
Subjects:
Animals
Antibody Formation - drug effects
Antibody Formation - immunology
Biological and medical sciences
Cell Line
Chitosan - administration & dosage
Chitosan - pharmacokinetics
Delayed-Action Preparations - administration & dosage
Delayed-Action Preparations - pharmacokinetics
DNA vaccine
Drug Delivery Systems - methods
Female
General pharmacology
Hepatitis B Vaccines - administration & dosage
Hepatitis B Vaccines - immunology
Hepatitis B Vaccines - pharmacokinetics
Hepatitis B virus
Immunogenicity
Mannose - administration & dosage
Mannose - immunology
Mannose - pharmacokinetics
Mannose-bearing Chitosan microspheres
Medical sciences
Mice
Mice, Inbred BALB C
Microspheres
PEI/DNA complexes
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Polyethyleneimine - administration & dosage
Polyethyleneimine - pharmacokinetics
T-Lymphocytes, Cytotoxic - drug effects
T-Lymphocytes, Cytotoxic - immunology
Target
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Vaccines, DNA - pharmacokinetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel approach involving the preparation of mannose-bearing chitosan microspheres with entrapping complexes of HBV DNA and PEI was developed to improve the delivery of DNA into antigen-presenting cells (APCs) after intramuscular (i.m.) injection. Compared with the traditional chitosan microspheres, the microspheres could quickly release intact and penetrative PEI/DNA complexes. What's more, chitosan was modified with mannose to target the primary APCs such as dendritic cells (DCs) owing to the high density of mannose receptors expressing on the surface of immature DCs. After i.m. immunization, the microspheres induced significantly enhanced serum antibody and cytotoxic T lymphocyte (CTL) responses in comparison to naked DNA.
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2007.05.018