Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses

Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus Morbillivirus in the P...

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Bibliographic Details
Published in:Vaccine 2003-07, Vol.21 (23), p.3282-3289
Main Authors: Khandelwal, Abha, Lakshmi Sita, G., Shaila, M.S.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Administration, Oral
Animals
Antibodies, Viral - biosynthesis
Antibody Formation - immunology
Antigens, Viral - biosynthesis
Antigens, Viral - immunology
Arachis - chemistry
Arachis - genetics
Arachis hypogaea
Biological and medical sciences
Cattle
Cell Division - physiology
Chlorocebus aethiops
Fundamental and applied biological sciences. Psychology
Hemagglutinin
Hemagglutinins - administration & dosage
Hemagglutinins - immunology
Immunization
Immunization Schedule
Immunohistochemistry
Lymphocytes - drug effects
Medical research
Microbiology
Neutralization
Neutralization Tests
Oral immunogenicity
Plants, Genetically Modified
Rinderpest virus
Rinderpest virus - immunology
Rodents
Ruminantia
Transgenic peanut
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - immunology
Vero Cells
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Virology
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Summary:Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus Morbillivirus in the Paramyxoviridae family. We have generated transgenic peanut ( Arachis hypogea L.) plants expressing hemagglutinin protein of RPV and report here, the induction of immune responses in cattle following oral feeding with transgenic leaves expressing hemagglutinin protein without oral adjuvant. Hemagglutinin-specific antibody was detected in the serum as confirmed by immunohistochemical staining of virus-infected cells, and in vitro neutralization of virus infectivity. Oral delivery also resulted in cell-mediated immune responses.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00192-0