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Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses
Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus Morbillivirus in the P...
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Published in: | Vaccine 2003-07, Vol.21 (23), p.3282-3289 |
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creator | Khandelwal, Abha Lakshmi Sita, G. Shaila, M.S. |
description | Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus
Morbillivirus in the
Paramyxoviridae family. We have generated transgenic peanut (
Arachis hypogea L.) plants expressing hemagglutinin protein of RPV and report here, the induction of immune responses in cattle following oral feeding with transgenic leaves expressing hemagglutinin protein without oral adjuvant. Hemagglutinin-specific antibody was detected in the serum as confirmed by immunohistochemical staining of virus-infected cells, and in vitro neutralization of virus infectivity. Oral delivery also resulted in cell-mediated immune responses. |
doi_str_mv | 10.1016/S0264-410X(03)00192-0 |
format | article |
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Morbillivirus in the
Paramyxoviridae family. We have generated transgenic peanut (
Arachis hypogea L.) plants expressing hemagglutinin protein of RPV and report here, the induction of immune responses in cattle following oral feeding with transgenic leaves expressing hemagglutinin protein without oral adjuvant. Hemagglutinin-specific antibody was detected in the serum as confirmed by immunohistochemical staining of virus-infected cells, and in vitro neutralization of virus infectivity. Oral delivery also resulted in cell-mediated immune responses.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(03)00192-0</identifier><identifier>PMID: 12804859</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic ; Administration, Oral ; Animals ; Antibodies, Viral - biosynthesis ; Antibody Formation - immunology ; Antigens, Viral - biosynthesis ; Antigens, Viral - immunology ; Arachis - chemistry ; Arachis - genetics ; Arachis hypogaea ; Biological and medical sciences ; Cattle ; Cell Division - physiology ; Chlorocebus aethiops ; Fundamental and applied biological sciences. Psychology ; Hemagglutinin ; Hemagglutinins - administration & dosage ; Hemagglutinins - immunology ; Immunization ; Immunization Schedule ; Immunohistochemistry ; Lymphocytes - drug effects ; Medical research ; Microbiology ; Neutralization ; Neutralization Tests ; Oral immunogenicity ; Plants, Genetically Modified ; Rinderpest virus ; Rinderpest virus - immunology ; Rodents ; Ruminantia ; Transgenic peanut ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, Synthetic - immunology ; Vero Cells ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Virology</subject><ispartof>Vaccine, 2003-07, Vol.21 (23), p.3282-3289</ispartof><rights>2003 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Elsevier Limited Jul 4, 2003</rights><rights>Copyright © 2003 Elsevier Science Ltd. All rights reserved. 2003 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-61d07038c9d86f392af08695a706ea20a70823e5710a1007659b29c17f3dc85c3</citedby><cites>FETCH-LOGICAL-c554t-61d07038c9d86f392af08695a706ea20a70823e5710a1007659b29c17f3dc85c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14891730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12804859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khandelwal, Abha</creatorcontrib><creatorcontrib>Lakshmi Sita, G.</creatorcontrib><creatorcontrib>Shaila, M.S.</creatorcontrib><title>Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus
Morbillivirus in the
Paramyxoviridae family. We have generated transgenic peanut (
Arachis hypogea L.) plants expressing hemagglutinin protein of RPV and report here, the induction of immune responses in cattle following oral feeding with transgenic leaves expressing hemagglutinin protein without oral adjuvant. Hemagglutinin-specific antibody was detected in the serum as confirmed by immunohistochemical staining of virus-infected cells, and in vitro neutralization of virus infectivity. Oral delivery also resulted in cell-mediated immune responses.</description><subject>Adjuvants, Immunologic</subject><subject>Administration, Oral</subject><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibody Formation - immunology</subject><subject>Antigens, Viral - biosynthesis</subject><subject>Antigens, Viral - immunology</subject><subject>Arachis - chemistry</subject><subject>Arachis - genetics</subject><subject>Arachis hypogaea</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Division - physiology</subject><subject>Chlorocebus aethiops</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutinin</subject><subject>Hemagglutinins - administration & dosage</subject><subject>Hemagglutinins - immunology</subject><subject>Immunization</subject><subject>Immunization Schedule</subject><subject>Immunohistochemistry</subject><subject>Lymphocytes - drug effects</subject><subject>Medical research</subject><subject>Microbiology</subject><subject>Neutralization</subject><subject>Neutralization Tests</subject><subject>Oral immunogenicity</subject><subject>Plants, Genetically Modified</subject><subject>Rinderpest virus</subject><subject>Rinderpest virus - immunology</subject><subject>Rodents</subject><subject>Ruminantia</subject><subject>Transgenic peanut</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Vero Cells</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFksuKFTEQhhtRnOPoIygBUcZFa6XTuW0UGbzBwCxUcBcy6epzMvRJ9yTp4-UVfGlzLsyoC10FKl_9-VP1V9VDCs8pUPHiIzSirVsKX06APQOguqnhVrWgSrK64VTdrhbXyFF1L6VLAOCM6rvVEW0UtIrrRfXzPNqB-PV6Dv6HzX4MZOyJszkPSL76vCIrXNvlcpizDz6QKY4Z_Q6KPnQYJ0yZbHycE8FvU8SUsCMFyNGGtMTgHZnQhjmXYjc7TCRN6Hxf6rtXkZSeaQwJ0_3qTm-HhA8O53H1-e2bT6fv67Pzdx9OX5_VjvM214J2IIEppzsleqYb24MSmlsJAm0D5VQNQy4pWAogBdcXjXZU9qxzijt2XL3c607zxRo7h6GYHcwU_drG72a03vx5E_zKLMeNkbQRum2KwNODQByv5jIAs_bJ4TDYgOOcjGRMSk11AU_-CVJe_EmmuCro47_Qy3GOocyhUK0QggOFQvE95eKYUsT-2jUFs82F2eXCbJdugJldLsy279HvX77pOgShAE8OgE3ODn3ZnvPphmuVppJthV7tOSwL2niMJjmPwWHnI7psutH_x8ovPFLX5g</recordid><startdate>20030704</startdate><enddate>20030704</enddate><creator>Khandelwal, Abha</creator><creator>Lakshmi Sita, G.</creator><creator>Shaila, M.S.</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><general>Elsevier Science Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030704</creationdate><title>Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses</title><author>Khandelwal, Abha ; Lakshmi Sita, G. ; Shaila, M.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-61d07038c9d86f392af08695a706ea20a70823e5710a1007659b29c17f3dc85c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adjuvants, Immunologic</topic><topic>Administration, Oral</topic><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibody Formation - immunology</topic><topic>Antigens, Viral - biosynthesis</topic><topic>Antigens, Viral - immunology</topic><topic>Arachis - chemistry</topic><topic>Arachis - genetics</topic><topic>Arachis hypogaea</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Division - physiology</topic><topic>Chlorocebus aethiops</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khandelwal, Abha</au><au>Lakshmi Sita, G.</au><au>Shaila, M.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2003-07-04</date><risdate>2003</risdate><volume>21</volume><issue>23</issue><spage>3282</spage><epage>3289</epage><pages>3282-3289</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Rinderpest is an acute, highly contagious often fatal disease of large and small ruminants, both domestic and wild. Global eradication of rinderpest needs a robust, safe and cost-effective vaccine. The causative agent, rinderpest virus (RPV) is an important member of the genus
Morbillivirus in the
Paramyxoviridae family. We have generated transgenic peanut (
Arachis hypogea L.) plants expressing hemagglutinin protein of RPV and report here, the induction of immune responses in cattle following oral feeding with transgenic leaves expressing hemagglutinin protein without oral adjuvant. Hemagglutinin-specific antibody was detected in the serum as confirmed by immunohistochemical staining of virus-infected cells, and in vitro neutralization of virus infectivity. Oral delivery also resulted in cell-mediated immune responses.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12804859</pmid><doi>10.1016/S0264-410X(03)00192-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adjuvants, Immunologic Administration, Oral Animals Antibodies, Viral - biosynthesis Antibody Formation - immunology Antigens, Viral - biosynthesis Antigens, Viral - immunology Arachis - chemistry Arachis - genetics Arachis hypogaea Biological and medical sciences Cattle Cell Division - physiology Chlorocebus aethiops Fundamental and applied biological sciences. Psychology Hemagglutinin Hemagglutinins - administration & dosage Hemagglutinins - immunology Immunization Immunization Schedule Immunohistochemistry Lymphocytes - drug effects Medical research Microbiology Neutralization Neutralization Tests Oral immunogenicity Plants, Genetically Modified Rinderpest virus Rinderpest virus - immunology Rodents Ruminantia Transgenic peanut Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - immunology Vero Cells Viral Vaccines - administration & dosage Viral Vaccines - immunology Virology |
title | Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses |
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