Proanthocyanidins Ameliorated Deficits of Lipid Metabolism in Type 2 Diabetes Mellitus Via Inhibiting Adipogenesis and Improving Mitochondrial Function

• Published in: International journal of molecular sciences 2020-03, Vol.21 (6), p.2029
• Main Authors: Tie, Fangfang, Wang, Jifei, Liang, Yuexin, Zhu, Shujun, Wang, Zhenhua, Li, Gang, Wang, Honglun
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Adipocytes; Adipogenesis; Adipogenesis - drug effects; Animals; Biflavonoids; Body Weight - drug effects; Catechin; Chinese medicine; Cholesterol; Dexamethasone; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - metabolism; Diet, High-Fat - adverse effects; Disease Models, Animal; Fatty acids; Fatty Liver; Flavonoids; Flavonoids - antagonists & inhibitors; Flavonoids - chemistry; High fat diet; Hyperglycemia; Insulin; Insulin Resistance; Lipid metabolism; Lipid Metabolism - drug effects; Lipids; Liver; Male; Membrane potential; Membrane Potential, Mitochondrial - drug effects; Metabolism; Mice; Mice, Inbred ICR; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Oxidation; Phosphorylation; Proanthocyanidins; Proanthocyanidins - chemistry; Proanthocyanidins - pharmacology; Proteins; Reactive Oxygen Species; Steatosis; Streptozocin; Streptozocin - adverse effects; Therapeutic applications
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21062029

Proanthocyanidins are the major active compounds extracted from Pall. var. (Fisch.) Koidz (I. ). Proanthocyanidins exhibit a variety of pharmacological activities such as anti-oxidation, anti-inflammation, anti-tumor, and lowering blood lipids. However, the underlying mechanism of its regulating effect on lipid metabolism in diabetic conditions remains unclear. The present study investigated the effects of . -derived proanthocyanidins on lipid metabolism in mice of type 2 diabetes mellitus (T2DM). Results demonstrated a beneficial effect of total proanthocyanidins on dysregulated lipid metabolism and hepatic steatosis in high-fat-diet/streptozocin (STZ)-induced T2DM. To identify the mechanisms, six flavan-3-ols were isolated from proanthocyanidins of . and their effects on adipogenesis and dexamethasone (Dex)-induced mitochondrial dysfunctions in 3T3-L1 adipocytes were determined. In vitro studies showed flavan-3-ols inhibited adipogenesis and restored mitochondrial function after Dex-induced insulin resistance, being suggested by increased mitochondrial membrane potential, intracellular ATP contents, mitochondrial mass and mitochondrial biogenesis, and reduced reactive oxygen species. Among the six flavan-3-ols, procyanidin B3 and procyanidin B1 exhibited the strongest effects. Our study suggests potential of proanthocyanidins as therapeutic target for diabetes.