Intraspecific Genomic Divergence and Minor Structural Variations in Leishmania (Viannia) panamensis

is one of the most important species associated with cutaneous leishmaniasis (CL) in Latin America. Despite its wide geographic distribution and pathogenic potential in humans and animals, the genomic variability of this species is low compared with other species circulating in the same geographical...

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Bibliographic Details
Published in:Genes 2020-02, Vol.11 (3), p.252
Main Authors: Patino, Luz H, Muñoz, Marina, Muskus, Carlos, Méndez, Claudia, Ramírez, Juan David
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Animals
Colombia - epidemiology
DNA Copy Number Variations - genetics
Female
Genetic Variation - genetics
Genome - genetics
Genomic Structural Variation - genetics
Genomics
Humans
Leishmania - genetics
Leishmania - pathogenicity
Leishmaniasis, Cutaneous - epidemiology
Leishmaniasis, Cutaneous - genetics
Leishmaniasis, Cutaneous - parasitology
Male
Middle Aged
Panama - epidemiology
Phylogeny
Polymorphism, Single Nucleotide - genetics
South America - epidemiology
Young Adult
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Summary:is one of the most important species associated with cutaneous leishmaniasis (CL) in Latin America. Despite its wide geographic distribution and pathogenic potential in humans and animals, the genomic variability of this species is low compared with other species circulating in the same geographical area. No studies have reported a detailed analysis of the whole genome of from clinical isolates using DNA high-throughput sequencing to clarify its intraspecific genomic variability or plausible divergence. Therefore, this study aimed to evaluate the intraspecific genomic variability of from Colombia and Panama. A total of 22 genomes were analyzed, 19 from Colombian patients with CL and three genomes from Panama obtained from public databases. The phylogenomic analysis revealed the potential existence of three well-supported clades as evidence of intraspecific divergence. Additionally, the whole-genome analysis showed low structural variations in terms of ploidy, copy number variations, and single-nucleotide polymorphisms (SNPs). SNPs shared among all clades were identified, revealing their importance in different biological processes of . The findings not only expand our knowledge of intraspecific genomic variability of one of the most important species in South America but also highlights the possible existence of different clades/lineages/subpopulations across a geographic scale.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes11030252