Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango ( Mangifera indica L.)-Based Bar Indigestible Fraction

• Published in: Nutrients 2020-03, Vol.12 (3), p.683
• Main Authors: Gutiérrez-Sarmiento, Wilbert, Sáyago-Ayerdi, Sonia Guadalupe, Goñi, Isabel, Gutiérrez-Miceli, Federico Antonio, Abud-Archila, Miguel, Rejón-Orantes, José Del Carmen, Rincón-Rosales, Reiner, Peña-Ocaña, Betsy Anaid, Ruíz-Valdiviezo, Víctor Manuel
• Format: Article
• Language: English
• Subjects: Adult; Anaerobic conditions; Bacteria - classification; Bacteria - growth & development; Batch culture; Carbohydrate metabolism; Carbohydrates; Colon; Colon - metabolism; Colon - microbiology; Diet; Dietary fiber; Dietary Fiber - administration & dosage; Dietary Fiber - metabolism; Digestion; Dysbacteriosis; Fecal microflora; Feces; Female; Fermentation; Fruits; Gastrointestinal Microbiome; Humans; Intestinal microflora; Intestine; Investigations; Large intestine; Male; Mangifera; Mangifera indica; Mangoes; Metabolic Networks and Pathways; Metabolic pathways; Metabolism; Metabolites; Microbiota; Microorganisms; Phenolic compounds; Phenols; Physiology; Phytochemicals; rRNA 16S
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12030683

Mango ( L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal microbiota during colonic fermentation is unknown. In this study, the isolated IF of a novel 'Ataulfo' mango-based bar (snack) UV-C irradiated and non-irradiated (UVMangoB and MangoB) were fermented. Colonic fermentation occurred in vitro under chemical-enzymatic, semi-anaerobic, batch culture and controlled pH colonic conditions. Changes in the structure of fecal microbiota were analyzed by 16s rRNA gene Illumina MiSeq sequencing. The community´s functional capabilities were determined in silico. The MangoB and UVMangoB increased the presence of , , , , , , , , , and genera. The alpha indexes showed a decrease in microbial diversity after 6 h of colonic fermentation. The coordinates analysis indicated any differences between irradiated and non-irradiated bar. The metabolic prediction demonstrated that MangoB and UVMangoB increase the microbiota carbohydrate metabolism pathway. This study suggests that IF of mango-based bar induced beneficial changes on microbial ecology and metabolic pathway that could be promissory to prevention or treatment of metabolic dysbiosis. However, in vivo interventions are necessary to confirm the interactions between microbiota modulating and intestinal beneficial effects.