Diversity of Metal-Resistant and Tensoactive-Producing Culturable Heterotrophic Bacteria Isolated from a Copper Mine in Brazilian Amazonia

• Published in: Scientific reports 2020-04, Vol.10 (1), p.6171, Article 6171
• Main Authors: Domingues, Vitor Sousa, de Souza Monteiro, Andrea, Júlio, Aline Daniela Lopes, Queiroz, Ana Luiza Lemos, dos Santos, Vera Lúcia
• Format: Article
• Language: English
• Subjects: 631/326/171; 704/158/855; Alcaligenes; Arsenic; Arsenic - adverse effects; Arsenic - metabolism; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Biodegradation, Environmental; Biodiversity; Bioremediation; Biotechnology; Brazil; Cadmium; Cadmium - adverse effects; Cadmium - metabolism; Copper; Copper - metabolism; Environmental Pollutants - adverse effects; Environmental Pollutants - metabolism; Environmental Pollution - prevention & control; Extracellular Polymeric Substance Matrix - metabolism; Genera; Heavy metals; Heterotrophic bacteria; Heterotrophic Processes; Humanities and Social Sciences; Hydrophobicity; Microbiota; Mining; multidisciplinary; Proteobacteria; RNA, Ribosomal, 16S; Science; Science (multidisciplinary); Soil Microbiology; Solubilization; Strains (organisms); Surface tension; Zinc - adverse effects; Zinc - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-62780-8

Bacterial extracellular polymeric substances (EPSs) present diverse properties of biotechnological interest, such as surface modification, metal adsorption and hydrophobic substances solubilization through surface tension reduction. Thus, there is a growing demand for new producing strains and structurally variable biomolecules with different properties. One approach for scanning this biodiversity consists of exploring environments under selective pressures. The aim of this study was to evaluate the composition of culturable heterotrophic bacterial communities from five different sites from a copper mine in the Amazon biome by an enrichment technique to obtain metal resistant bacteria (lead, arsenic, cadmium, copper and zinc) capable of producing EPSs. The bacterial densities at the sites varied from 2.42 × 10 3 to 1.34 × 10 8 NMP mL −1 and the 77 bacterial isolates obtained were classified in four divisions, β-Proteobacteria (16.88%), γ-Proteobacteria (7.29%), Firmicutes (61%) and Actinobacteria (12.98%). Bacillus , Alcaligenes , and Lysinibacillus were the most dominant among the 16 observed genera, but the relative frequency of each varied according to the sample and the metal used in the enrichment culture. 58% of the bacterial strains (45) could produce EPSs. From these, 33 strains showed emulsifying activity (E 24 ), and 9 of them reached values higher than 49%. Only Actinomyces viscosus E3.Pb5 and Bacillus subtilis group E3.As2 reduced the medium surface tension to values lower than 35 mN m −1 . It was possible to confirm the high presence of bacteria capable of producing EPSs with tensoactive properties in Amazon copper mines and the evolutionary pressure exerted by the heavy metals during enrichment. These molecules can be tested as an alternative for use in processes that involve the removal of metals, such as the bioremediation of contaminated environments.