Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women

• Published in: Scientific reports 2020-04, Vol.10 (1), p.6515, Article 6515
• Main Authors: Nicoletti, C. F., Pinhel, M. A. S., Noronha, N. Y., de Oliveira, B. A., Salgado Junior, W., Jácome, A., Diaz-Lagares, A., Casanueva, F., Crujeiras, A. B., Nonino, C. B.
• Format: Article
• Language: English
• Subjects: 38; 38/39; 38/61; 38/90; 45; 45/22; 45/47; 692/4017; 692/53/2423; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Adipogenesis; Adipose tissue; Adiposity - genetics; Adult; Aminopeptidases - genetics; Aminopeptidases - metabolism; Bariatric Surgery; Butyrate Response Factor 1 - genetics; Butyrate Response Factor 1 - metabolism; CpG islands; Deoxyribonucleic acid; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases - genetics; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases - metabolism; DNA; DNA Methylation; DNA probes; Female; Gastrointestinal surgery; Gene expression; Gene silencing; Genetic factors; Genomes; Humanities and Social Sciences; Humans; Insulin; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; MAP kinase; Membrane Proteins - genetics; Membrane Proteins - metabolism; Metabolic Networks and Pathways; Middle Aged; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; multidisciplinary; NF-κB protein; Obesity; Obesity - genetics; Obesity - metabolism; Obesity - surgery; Oxidative metabolism; Oxidative stress; Postoperative Period; Preoperative Period; Proteolysis; Science; Science (multidisciplinary); Serine Endopeptidases - genetics; Serine Endopeptidases - metabolism; Surgery; Transcriptome; Ubiquitin Thiolesterase - genetics; Ubiquitin Thiolesterase - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-60814-9

DNA methylation could provide a link between environmental, genetic factors and weight control and can modify gene expression pattern. This study aimed to identify genes, which are differentially expressed and methylated depending on adiposity state by evaluating normal weight women and obese women before and after bariatric surgery (BS). We enrolled 24 normal weight (BMI: 22.5 ± 1.6 kg/m 2 ) and 24 obese women (BMI: 43.3 ± 5.7 kg/m 2 ) submitted to BS. Genome-wide methylation analysis was conducted using Infinium Human Methylation 450 BeadChip (threshold for significant CpG sites based on delta methylation level with a minimum value of 5%, a false discovery rate correction (FDR) of q