Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia

• Published in: Journal of medical virology 2016-07, Vol.88 (7), p.1173-1179
• Main Authors: Luchsinger, Vivian, Prades, Yara, Ruiz, Mauricio, Pizarro, Rolando, Rossi, Patricio, Lizama, Luis, Garmendia, María Luisa, Meza, Angela, Larrañaga, Carmen, Avendaño, Luis F.
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Community-Acquired Infections - diagnosis; Community-Acquired Infections - virology; community-acquired pneumonia; diagnosis; Epidemiology; Female; Fluorescent Antibody Technique, Indirect; Hospitalization; Humans; Immunoassay; Luminex xTAG RVP; Male; Medical screening; Middle Aged; Molecular Diagnostic Techniques - methods; Nasopharynx - virology; Pneumonia; Pneumonia, Viral - diagnosis; Pneumonia, Viral - virology; Polymerase chain reaction; Reagent Kits, Diagnostic; Real-Time Polymerase Chain Reaction - methods; respiratory viruses; Sensitivity and Specificity; Viral infections; Virology; Viruses - isolation & purification; Young Adult
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.24463

Community‐acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT‐PCR (rtRT‐PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT‐PCR (P = 0.3), and 2.7% for IFA (2.7%) (P