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Regenerative therapy by endometrial mesenchymal stem cells in thin endometrium with repeated implantation failure. A novel strategy
Our primary objective was to evaluate the endometrial changes before and after the transfer of endometrial mesenchymal stem cells (enMSCs) in a population of thinned endometrium women, with absence or hypo-responsiveness to estrogen and repeated implantation failure (RIF). The secondary objective wa...
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Published in: | JBRA assisted reproduction 2020, Vol.24 (2), p.118-127 |
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creator | Tersoglio, Alberto E Tersoglio, Sebastian Salatino, Dante R Castro, Matías Gonzalez, Adriana Hinojosa, Mariana Castellano, Onias |
description | Our primary objective was to evaluate the endometrial changes before and after the transfer of endometrial mesenchymal stem cells (enMSCs) in a population of thinned endometrium women, with absence or hypo-responsiveness to estrogen and repeated implantation failure (RIF). The secondary objective was to evaluate the clinical outcomes of the intervention in terms of clinical pregnancy (CP), early abortions, ongoing pregnancy and live birth delivery rate (LBDR) per in vitro fertilization (IVF) cycle.
A longitudinal and experimental study. The intervention was defined as "subendometrial inoculation of enMSCs," and the post-intervention changes were evaluated by the following variables: endometrial thickness (Eth), endometrial flow cytometry (enFC), endometrial histopathology (enHP) and endometrial immunohistochemistry (enIHQ). The variables were analyzed after the intervention (Post-treatment) regarding previous values (Pretreatment).
Eth values before and after treatment with enMSCs were 5.24±1.24 mm vs. 9.93±0.77 (p=0.000), respectively. Endometrial Flow Cytometry showed significant differences in favor of Normalized variables in the post-treatment assessment, associated with the pretreatment, LT/Li, LB/Li, NK/Li, CD8/CD3+ and CD4/CD8 (p≤0.015), respectively. Only two variables Li/PC and CD4/CD3 had NS (p=0.167 and 0.118). A similar analysis was performed on enHP with an HP increase post-treatment (p=0.007). The CP rate was 79.31% (23/29), a live birth delivery rate per embryo transfer was 45.45% (10/22) and ongoing pregnancy 7/29 (24.14%).
Subendometrial enMSCs inoculation produces a significant increase in endometrial thickness; normalize the enHP, enIHQ and enFC. As a result, IVF after treatment with enMSCs yields a higher rate of CP and LBDR. |
doi_str_mv | 10.5935/1518-0557.20190061 |
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A longitudinal and experimental study. The intervention was defined as "subendometrial inoculation of enMSCs," and the post-intervention changes were evaluated by the following variables: endometrial thickness (Eth), endometrial flow cytometry (enFC), endometrial histopathology (enHP) and endometrial immunohistochemistry (enIHQ). The variables were analyzed after the intervention (Post-treatment) regarding previous values (Pretreatment).
Eth values before and after treatment with enMSCs were 5.24±1.24 mm vs. 9.93±0.77 (p=0.000), respectively. Endometrial Flow Cytometry showed significant differences in favor of Normalized variables in the post-treatment assessment, associated with the pretreatment, LT/Li, LB/Li, NK/Li, CD8/CD3+ and CD4/CD8 (p≤0.015), respectively. Only two variables Li/PC and CD4/CD3 had NS (p=0.167 and 0.118). A similar analysis was performed on enHP with an HP increase post-treatment (p=0.007). The CP rate was 79.31% (23/29), a live birth delivery rate per embryo transfer was 45.45% (10/22) and ongoing pregnancy 7/29 (24.14%).
Subendometrial enMSCs inoculation produces a significant increase in endometrial thickness; normalize the enHP, enIHQ and enFC. As a result, IVF after treatment with enMSCs yields a higher rate of CP and LBDR.</description><identifier>ISSN: 1518-0557</identifier><identifier>ISSN: 1517-5693</identifier><identifier>EISSN: 1518-0557</identifier><identifier>DOI: 10.5935/1518-0557.20190061</identifier><identifier>PMID: 31589391</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</publisher><subject>Angiogenesis ; Cytokines ; Embryos ; Endometrium ; Original ; Patients ; Pregnancy ; Quality ; Ultrasonic imaging ; Vascular endothelial growth factor</subject><ispartof>JBRA assisted reproduction, 2020, Vol.24 (2), p.118-127</ispartof><rights>2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-98eacb6d09350396cb707b39445d680db65d5833ea89098f501afa47b1de27403</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2396849622/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2396849622?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,25753,27923,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31589391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tersoglio, Alberto E</creatorcontrib><creatorcontrib>Tersoglio, Sebastian</creatorcontrib><creatorcontrib>Salatino, Dante R</creatorcontrib><creatorcontrib>Castro, Matías</creatorcontrib><creatorcontrib>Gonzalez, Adriana</creatorcontrib><creatorcontrib>Hinojosa, Mariana</creatorcontrib><creatorcontrib>Castellano, Onias</creatorcontrib><title>Regenerative therapy by endometrial mesenchymal stem cells in thin endometrium with repeated implantation failure. A novel strategy</title><title>JBRA assisted reproduction</title><addtitle>JBRA Assist Reprod</addtitle><description>Our primary objective was to evaluate the endometrial changes before and after the transfer of endometrial mesenchymal stem cells (enMSCs) in a population of thinned endometrium women, with absence or hypo-responsiveness to estrogen and repeated implantation failure (RIF). The secondary objective was to evaluate the clinical outcomes of the intervention in terms of clinical pregnancy (CP), early abortions, ongoing pregnancy and live birth delivery rate (LBDR) per in vitro fertilization (IVF) cycle.
A longitudinal and experimental study. The intervention was defined as "subendometrial inoculation of enMSCs," and the post-intervention changes were evaluated by the following variables: endometrial thickness (Eth), endometrial flow cytometry (enFC), endometrial histopathology (enHP) and endometrial immunohistochemistry (enIHQ). The variables were analyzed after the intervention (Post-treatment) regarding previous values (Pretreatment).
Eth values before and after treatment with enMSCs were 5.24±1.24 mm vs. 9.93±0.77 (p=0.000), respectively. Endometrial Flow Cytometry showed significant differences in favor of Normalized variables in the post-treatment assessment, associated with the pretreatment, LT/Li, LB/Li, NK/Li, CD8/CD3+ and CD4/CD8 (p≤0.015), respectively. Only two variables Li/PC and CD4/CD3 had NS (p=0.167 and 0.118). A similar analysis was performed on enHP with an HP increase post-treatment (p=0.007). The CP rate was 79.31% (23/29), a live birth delivery rate per embryo transfer was 45.45% (10/22) and ongoing pregnancy 7/29 (24.14%).
Subendometrial enMSCs inoculation produces a significant increase in endometrial thickness; normalize the enHP, enIHQ and enFC. As a result, IVF after treatment with enMSCs yields a higher rate of CP and LBDR.</description><subject>Angiogenesis</subject><subject>Cytokines</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Original</subject><subject>Patients</subject><subject>Pregnancy</subject><subject>Quality</subject><subject>Ultrasonic imaging</subject><subject>Vascular endothelial growth factor</subject><issn>1518-0557</issn><issn>1517-5693</issn><issn>1518-0557</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkUFr3DAQhUVpaUKaP9BDEfTSy25HliVLl0IIbRMIFEpyFrI93lWwZVeSt_icPx6ZJEvaizRI3zzN0yPkI4Ot0Fx8ZYKpDQhRbQtgGkCyN-T0ePj2VX1CzmO8B8gYK3gJ78kJZ0JprtkpefiNO_QYbHIHpGmfq2mh9ULRt-OAKTjb0wEj-ma_DLmOCQfaYN9H6nxuyMsRnQf616U9DTihTdhSN0y99SmLj5521vVzwC29oH484CqVn8Xd8oG862wf8fx5PyN3P77fXl5tbn79vL68uNk0JYe00QptU8sWsn3gWjZ1BVXNdVmKVipoaylaoThHqzRo1QlgtrNlVbMWi6oEfka-PelOcz1g26DPA_RmCm6wYTGjdebfG-_2ZjceTMWk1qCywJdngTD-mTEmM7i4_oX1OM7RFByKsmJMyYx-_g-9H-fgs71MaalKLYsiU8UT1YQxxoDdcRgGZo3ZrCmaNUXzEnNu-vTaxrHlJVT-CJnupU0</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Tersoglio, Alberto E</creator><creator>Tersoglio, Sebastian</creator><creator>Salatino, Dante R</creator><creator>Castro, Matías</creator><creator>Gonzalez, Adriana</creator><creator>Hinojosa, Mariana</creator><creator>Castellano, Onias</creator><general>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</general><general>Brazilian Society of Assisted Reproduction</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2020</creationdate><title>Regenerative therapy by endometrial mesenchymal stem cells in thin endometrium with repeated implantation failure. A novel strategy</title><author>Tersoglio, Alberto E ; Tersoglio, Sebastian ; Salatino, Dante R ; Castro, Matías ; Gonzalez, Adriana ; Hinojosa, Mariana ; Castellano, Onias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-98eacb6d09350396cb707b39445d680db65d5833ea89098f501afa47b1de27403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angiogenesis</topic><topic>Cytokines</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Original</topic><topic>Patients</topic><topic>Pregnancy</topic><topic>Quality</topic><topic>Ultrasonic imaging</topic><topic>Vascular endothelial growth factor</topic><toplevel>online_resources</toplevel><creatorcontrib>Tersoglio, Alberto E</creatorcontrib><creatorcontrib>Tersoglio, Sebastian</creatorcontrib><creatorcontrib>Salatino, Dante R</creatorcontrib><creatorcontrib>Castro, Matías</creatorcontrib><creatorcontrib>Gonzalez, Adriana</creatorcontrib><creatorcontrib>Hinojosa, Mariana</creatorcontrib><creatorcontrib>Castellano, Onias</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>JBRA assisted reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tersoglio, Alberto E</au><au>Tersoglio, Sebastian</au><au>Salatino, Dante R</au><au>Castro, Matías</au><au>Gonzalez, Adriana</au><au>Hinojosa, Mariana</au><au>Castellano, Onias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regenerative therapy by endometrial mesenchymal stem cells in thin endometrium with repeated implantation failure. A novel strategy</atitle><jtitle>JBRA assisted reproduction</jtitle><addtitle>JBRA Assist Reprod</addtitle><date>2020</date><risdate>2020</risdate><volume>24</volume><issue>2</issue><spage>118</spage><epage>127</epage><pages>118-127</pages><issn>1518-0557</issn><issn>1517-5693</issn><eissn>1518-0557</eissn><abstract>Our primary objective was to evaluate the endometrial changes before and after the transfer of endometrial mesenchymal stem cells (enMSCs) in a population of thinned endometrium women, with absence or hypo-responsiveness to estrogen and repeated implantation failure (RIF). The secondary objective was to evaluate the clinical outcomes of the intervention in terms of clinical pregnancy (CP), early abortions, ongoing pregnancy and live birth delivery rate (LBDR) per in vitro fertilization (IVF) cycle.
A longitudinal and experimental study. The intervention was defined as "subendometrial inoculation of enMSCs," and the post-intervention changes were evaluated by the following variables: endometrial thickness (Eth), endometrial flow cytometry (enFC), endometrial histopathology (enHP) and endometrial immunohistochemistry (enIHQ). The variables were analyzed after the intervention (Post-treatment) regarding previous values (Pretreatment).
Eth values before and after treatment with enMSCs were 5.24±1.24 mm vs. 9.93±0.77 (p=0.000), respectively. Endometrial Flow Cytometry showed significant differences in favor of Normalized variables in the post-treatment assessment, associated with the pretreatment, LT/Li, LB/Li, NK/Li, CD8/CD3+ and CD4/CD8 (p≤0.015), respectively. Only two variables Li/PC and CD4/CD3 had NS (p=0.167 and 0.118). A similar analysis was performed on enHP with an HP increase post-treatment (p=0.007). The CP rate was 79.31% (23/29), a live birth delivery rate per embryo transfer was 45.45% (10/22) and ongoing pregnancy 7/29 (24.14%).
Subendometrial enMSCs inoculation produces a significant increase in endometrial thickness; normalize the enHP, enIHQ and enFC. As a result, IVF after treatment with enMSCs yields a higher rate of CP and LBDR.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</pub><pmid>31589391</pmid><doi>10.5935/1518-0557.20190061</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Angiogenesis Cytokines Embryos Endometrium Original Patients Pregnancy Quality Ultrasonic imaging Vascular endothelial growth factor |
title | Regenerative therapy by endometrial mesenchymal stem cells in thin endometrium with repeated implantation failure. A novel strategy |
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