Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato

• Published in: Molecular plant pathology 2020-05, Vol.21 (5), p.636-651
• Main Authors: Liu, Hai‐Feng, Xue, Xiao‐Jing, Yu, Yue, Xu, Ming‐Ming, Lu, Chong‐Chong, Meng, Xuan‐Lin, Zhang, Bao‐Gang, Ding, Xin‐Hua, Chu, Zhao‐Hui
• Format: Article
• Language: English
• Subjects: ABA; ABA1; Abiotic stress; Abscisic acid; Biosynthesis; Blight; Copper; Copper compounds; Disease; Disease resistance; EIN3; ethylene; fungicide; Fungicides; Gene expression; Late blight; NCED1; Original; Pathogens; Physiological aspects; Phytophthora infestans; Plant diseases; potato late blight; Potatoes; Roles; Transcription; Vegetable industry; Vegetables
• ISSN: 1464-6722; 1364-3703
• DOI: 10.1111/mpp.12919

Copper‐based antimicrobial compounds are widely and historically used to control plant diseases, such as late blight caused by Phytophthora infestans, which seriously affects the yield and quality of potato. We previously identified that copper ion (Cu2+) acts as an extremely sensitive elicitor to induce ethylene (ET)‐dependent immunity in Arabidopsis. Here, we found that Cu2+ induces the defence response to P. infestans in potato. Cu2+ suppresses the transcription of the abscisic acid (ABA) biosynthetic genes StABA1 and StNCED1, resulting in decreased ABA content. Treatment with ABA or inhibitor fluridone made potato more susceptible or resistance to late blight, respectively. In addition, potato with knockdown of StABA1 or StNCED1 showed greater resistance to late blight, suggesting that ABA negatively regulates potato resistance to P. infestans. Cu2+ also promotes the rapid biosynthesis of ET. Potato plants treated with 1‐aminocyclopropane‐1‐carboxylate showed enhanced resistance to late blight. Repressed expression of StEIN2 or StEIN3 resulted in enhanced transcription of StABA1 and StNCED1, accumulation of ABA and susceptibility to P. infestans. Consistently, StEIN3 directly binds to the promoter regions of StABA1 and StNCED1. Overall, we concluded that Cu2+ triggers the defence response to potato late blight by activating ET biosynthesis to inhibit the biosynthesis of ABA. Cu2+‐mediated late blight resistance activates ET signalling, subsequently suppressing ABA biosynthesis. This recalls two tips when using copper‐based antimicrobial compounds: water in advance; avoid strong sunshine and drought.