Cytochrome P450 metabolic resistance (CYP6P9a) to pyrethroids imposes a fitness cost in the major African malaria vector Anopheles funestus

Metabolic resistance threatens the sustainability of pyrethroid-based malaria control interventions. Elucidating the fitness cost and potential reversal of metabolic resistance is crucial to design suitable resistance management strategies. Here, we deciphered the fitness cost associated with the CY...

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Bibliographic Details
Published in:Heredity 2020-05, Vol.124 (5), p.621-632
Main Authors: Tchouakui, Magellan, Riveron Miranda, Jacob, Mugenzi, Leon M J, Djonabaye, Doumani, Wondji, Murielle J, Tchoupo, Micareme, Tchapga, Williams, Njiokou, Flobert, Wondji, Charles S
Format: Article
Language:English
Subjects:
Anopheles funestus
Aquatic insects
Chi-square test
Culicidae
Cytochrome P450
Cytochromes P450
Eggs
Fecundity
Fitness
Genotypes
Genotyping
Heterozygotes
Impact resistance
Larvae
Larval development
Life span
Malaria
Metabolism
Mosquitoes
Pupation
Pyrethroids
Strains (organisms)
Vector-borne diseases
Vectors
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Summary:Metabolic resistance threatens the sustainability of pyrethroid-based malaria control interventions. Elucidating the fitness cost and potential reversal of metabolic resistance is crucial to design suitable resistance management strategies. Here, we deciphered the fitness cost associated with the CYP6P9a (P450-mediated metabolic resistance) in the major African malaria vector Anopheles funestus. Reciprocal crosses were performed between a pyrethroid susceptible (FANG) and resistant (FUMOZ-R) laboratory strains and the hybrid strains showed intermediate resistance. Genotyping the CYP6P9a-R resistance allele in oviposited females revealed that CYP6P9a negatively impacts the fecundity as homozygote susceptible mosquitoes (CYP6P9a-SS) lay more eggs than heterozygote (OR = 2.04: P = 0.01) and homozygote resistant mosquitoes. CYP6P9a also imposes a significant fitness cost on the larval development as homozygote resistant larvae (CYP6P9a-RR) developed significantly slower than heterozygote and homozygote susceptible mosquitoes (χ  = 11.2; P = 0.0008). This fitness cost was further supported by the late pupation of homozygote resistant than susceptible mosquitoes (OR = 2.50; P 
ISSN:0018-067X
1365-2540
DOI:10.1038/s41437-020-0304-1