MiR‐9 promotes angiogenesis of endothelial progenitor cell to facilitate thrombi recanalization via targeting TRPM7 through PI3K/Akt/autophagy pathway

Endothelial progenitor cells (EPCs) have emerged as a promising therapeutic choice for thrombi recanalization. However, this role of EPCs is confined by some detrimental factors. The aim of this study was to explore the role of the miR‐9‐5p in regulation of the proliferation, migration and angiogene...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2020-04, Vol.24 (8), p.4624-4632
Main Authors: Zhou, Dong‐Ming, Sun, Li‐Li, Zhu, Jian, Chen, Bing, Li, Xiao‐Qiang, Li, Wen‐Dong
Format: Article
Language:English
Subjects:
angiogenesis
Animals
Autophagy - genetics
Cell Movement - genetics
Cell Proliferation - genetics
endothelial progenitor cells
Endothelial Progenitor Cells - metabolism
Endothelial Progenitor Cells - pathology
Gene Expression Regulation - genetics
Humans
Mice
MicroRNAs - genetics
migration
miR‐9‐5p
Morphogenesis - genetics
Neoplasm Invasiveness - genetics
Neovascularization, Pathologic - genetics
Original
Phosphatidylinositol 3-Kinases - genetics
Protein-Serine-Threonine Kinases - genetics
Proto-Oncogene Proteins c-akt - genetics
thrombosis
Thrombosis - genetics
Thrombosis - pathology
Thrombosis - therapy
TRPM Cation Channels - genetics
TRPM7
Wound Healing - genetics
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Summary:Endothelial progenitor cells (EPCs) have emerged as a promising therapeutic choice for thrombi recanalization. However, this role of EPCs is confined by some detrimental factors. The aim of this study was to explore the role of the miR‐9‐5p in regulation of the proliferation, migration and angiogenesis of EPCs and the subsequent therapeutic role in thrombosis event. Wound healing, transwell assay, tube formation assay and in vivo angiogenesis assay were carried out to measure cell migration, invasion and angiogenic abilities, respectively. Western blot was performed to elucidate the relationship between miR‐9‐5p and TRPM7 in the autophagy pathway. It was found that miR‐9‐5p could promote migration, invasion and angiogenesis of EPCs by attenuating TRPM7 expression via activating PI3K/Akt/autophagy pathway. In conclusion, miR‐9‐5p, targets TRPM7 via the PI3K/Ak/autophagy pathway, thereby mediating cell proliferation, migration and angiogenesis in EPCs. Acting as a potential therapeutic target, miR‐9‐5p may play an important role in the prognosis of DVT.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.15124